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| Status |
Public on Oct 05, 2016 |
| Title |
Expression array of early postnatal Mt1-mmp-targeted mammary glands |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Mammary gland branching morphogenesis is thought to depend on the mobilization of the membrane-anchored matrix metalloproteinases, MT1-MMP and MT2-MMP, that drive epithelial cell invasion by remodeling the extracellular matrix and triggering associated signaling cascades. However, the roles that these proteinases play during mammary gland development in vivo remains undefined. A mammary gland branching program that occurs during the first 10 days of early postnatal development was used to characterize the impact of global Mt1-mmp or Mt2-mmp targeting on mammary gland morphogenesis.
Transcriptome profiling of ductal networks and associated stroma was used to investigate the functional roles of MT1-MMP in the early postnatal mammary gland in an unbiased fashion.
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| |
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| Overall design |
Intact mammary gland tissue was harvested from global knockout [Mt1-mmp(-/-)] mice and wild-type [Mt1-mmp(+/+)] littermates on an outbred Swiss Black background at postnatal day 10 for RNA extraction and hybridization on Affymetrix microarrays.
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| |
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| Contributor(s) |
Feinberg TY, Weiss SJ |
| Citation(s) |
27633994 |
| Submission date |
Feb 08, 2016 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Stephen J. Weiss |
| E-mail(s) |
sjweiss@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Life Sciences Institute
|
| Lab |
Stephen J. Weiss lab
|
| Street address |
210 Washtenaw Avenue
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (4)
|
| GSM2055982 |
Mt1-mmp(+/+) mammary gland, biological replicate 1 |
| GSM2055983 |
Mt1-mmp(+/+) mammary gland, biological replicate 2 |
| GSM2055984 |
Mt1-mmp(-/-) mammary gland, biological replicate 1 |
| GSM2055985 |
Mt1-mmp(-/-) mammary gland, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE77679 |
Affymetrix microarrays of early postnatal Mt1-mmp-targeted and Mt2-mmp-targeted mammary glands |
|
| Relations |
| BioProject |
PRJNA311199 |
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