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| Status |
Public on Jul 08, 2016 |
| Title |
Embryonic neocortical HuR-immunoprecipitated mRNAs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Forkhead-box domain (Fox) containing family members are known autism spectrum associated genes. Here we show that, within the developing neocortex, the distinct phospho-states of a single RNA-binding protein, Hu antigen R, dictate mRNA translation and are sufficient to define distinct Foxp-characterized subpopulations of neocortical projection neurons. This demonstrates the importance of HuR phospho-states within the framework of the developing brain and further confirms the role of mRNA translation in autism pathogenesis.
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| Overall design |
Fetal mouse neocortices were dissected, homogenized, and immunoprecipitated with either anti-HuR or IgG control antibodies. RNA was prepared from the immunoprecipitates and assayed on Affymetrix Mouse Exon microarrays.
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| Contributor(s) |
Hart RP, Rasin M |
| Citation(s) |
27383233 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 NS064303 |
Molecular Control of Corticospinal System Formation by Intermediate Targets |
UMDNJ-Robert Wood Johnson Medical School |
RASIN |
| R01 NS075367 |
Role of first neocortical RNA-operon in specification of neocortical projection n |
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY--RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL |
RASIN |
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| Submission date |
Feb 09, 2016 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Ronald P. Hart |
| E-mail(s) |
rhart@rutgers.edu
|
| Phone |
848-445-1783
|
| Organization name |
Rutgers University
|
| Department |
Cell Biology & Neuroscience
|
| Street address |
604 Allison Rd Rm B430
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA311269 |
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