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| Status |
Public on Feb 18, 2016 |
| Title |
The effects of RED GREEN and BLUE LED light on human normal healthy fibroblast cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The purpose of this study is to investigate whether LED light at different wavelength can improve wound healing in normal fibroblast. In this study, both diabetic and normal fibroblast cell lines were cultured and created some artificial wounds with pipette tips. After LED irradiation and microarray analysis, we found that GPCR Class A, a rhodopsin-like-structure gene, was significantly up regulated in all treated groups. Besides, for normal cell treated groups, the expression of other genes relevant to defense response to virus became strongly higher. However, in diabetic cells, genes relating to acute inflammatory response and mitotic cell cycle were highly expressed. Researchers also found that diabetic cells significantly respond to wound healing better than normal cells because inflammatory response, cell migration, cell proliferation, cell adhesion, and regulation of mitosis pathways can be instantly activated by red and green LED light according to gene expression profiles.
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| Overall design |
Normal cell lines were irradiated with different wavelength (red/green/blue) LED color and compare their gene expression with the control groups.
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| Contributor(s) |
Chotikasemsri P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 17, 2016 |
| Last update date |
Jan 09, 2018 |
| Contact name |
pongsathorn chotikasemsri |
| E-mail(s) |
golfgoofy19@gmail.com
|
| Phone |
+66898706878
|
| Organization name |
prince of songkhla university
|
| Department |
faculty of medicine
|
| Lab |
biomedical engineering
|
| Street address |
kajanavanich rd
|
| City |
hatyai |
| State/province |
songkhla |
| ZIP/Postal code |
90110 |
| Country |
Thailand |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (4)
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| GSM2064581 |
Normal_Healthy_Fibroblast_No_light_irradiation |
| GSM2064585 |
Normal_Healthy_Fibroblast_RED_light_irradiation_for_10_min |
| GSM2064586 |
Normal_Healthy_Fibroblast_GREEN_light_irradiation_for_10_min |
| GSM2064587 |
Normal_Healthy_Fibroblast_BLUE_light_irradiation_for_10_min |
|
| Relations |
| BioProject |
PRJNA312362 |
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