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Series GSE7810 Query DataSets for GSE7810
Status Public on May 18, 2007
Title Comparative analysis of gene expression WT and Nrf2-/- mice Type II cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We hypothesize that gene expression in the Type II cells of Nrf2+/+ and Nrf2-/- mice are divergent thus contributing the cell growth. More specifically, type II cells from Nrf2-/- mice have increased reactive oxygen species that cause the impaired cell growth. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between Nrf2+/+ and Nrf2-/- cells.
Keywords: comparative expression profiling
Overall design . This study utilizes microarray analysis to test these hypotheses. Three sets of type II cells were isolated from lungs from both Nrf2+/+ and Nrf2-/- mice and grown for 5 days. RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05. Further, Absolute | FC - FC SEM | >= 1.5.
Contributor(s) Scollick CM, Machireddy N, Kleeberger SR, Yamamoto M, Kensler TW, Biswal S, Reddy SP
Citation(s) 17895394
Submission date May 15, 2007
Last update date Feb 11, 2019
Contact name Narsa Reddy Machireddy
Phone 1-410-955-4711
Fax 1-410-955-0299
Organization name Bloomberg School of Public Health
Department Environmental Health Sciences
Lab Sekhar Reddy Lab
Street address 615, N Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM189472 MNR 1-2 WT 3
GSM189473 MNR 2-2 WT 4
GSM189476 MNR 5-2 KO 2
BioProject PRJNA100031

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Supplementary file Size Download File type/resource
GSE7810_RAW.tar 36.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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