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Status |
Public on May 18, 2007 |
Title |
Comparative analysis of gene expression WT and Nrf2-/- mice Type II cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
We hypothesize that gene expression in the Type II cells of Nrf2+/+ and Nrf2-/- mice are divergent thus contributing the cell growth. More specifically, type II cells from Nrf2-/- mice have increased reactive oxygen species that cause the impaired cell growth. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between Nrf2+/+ and Nrf2-/- cells. Keywords: comparative expression profiling
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Overall design |
. This study utilizes microarray analysis to test these hypotheses. Three sets of type II cells were isolated from lungs from both Nrf2+/+ and Nrf2-/- mice and grown for 5 days. RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05. Further, Absolute | FC - FC SEM | >= 1.5.
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Contributor(s) |
Scollick CM, Machireddy N, Kleeberger SR, Yamamoto M, Kensler TW, Biswal S, Reddy SP |
Citation(s) |
17895394 |
Submission date |
May 15, 2007 |
Last update date |
Feb 11, 2019 |
Contact name |
Narsa Reddy Machireddy |
E-mail(s) |
nmachire@jhsph.edu
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Phone |
1-410-955-4711
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Fax |
1-410-955-0299
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Organization name |
Bloomberg School of Public Health
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Department |
Environmental Health Sciences
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Lab |
Sekhar Reddy Lab
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Street address |
615, N Wolfe St
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City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21205 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (9)
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Relations |
BioProject |
PRJNA100031 |