NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE78149 Query DataSets for GSE78149
Status Public on Sep 24, 2018
Title Stella prevents excessive de novo DNA methylation during mouse oogenesis
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Oocyte acquires developmental competence during its maturation. This stage is accompanied with large-scale alteration in transcription, and series of genome-wide epigenetic reprogramming, including de novo establishment of DNA methylation. However, our understanding of mechanisms regulating this process is limited. To investigate the role of Stella (Dppa3) in de novo methylation during mouse oogenesis, here we measured DNA methylation by RRBS and expression profiles by RNA-seq in PGCs and oocytes at serveral development stages, including genotypes of both Stella (Dppa3) +/- and Stella -/-.
 
Overall design We measured DNA methylation by RRBS and expression profiles by RNA-seq in PGCs and oocytes at serveral development stages (E13.5, D5, D10, D20, MII), including genotypes of both Stella (Dppa3) +/- and Stella -/-. DNA methylation of sperm with both genotypes were also measured by RRBS for comparision.
 
Contributor(s) Li Y, Zhang Z, Chen J, Huang H, Li X, Xu S, Dong Q, Tan J, Zheng Y, Xu G, Wang Z, Wang H, Zhu B
Citation(s) 30487604
Submission date Feb 22, 2016
Last update date May 15, 2019
Contact name Zhuqiang Zhang
E-mail(s) zhangzhuqiang@ibp.ac.cn
Organization name Institute of Biophysics, CAS
Street address 15 Datun Road, Chaoyang District.
City Beijing
State/province ---
ZIP/Postal code 100101
Country China
 
Platforms (4)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (83)
GSM2068220 MII-oocyte-Stella-Het-RRBS.rep2
GSM2068221 E13.5-Male-PGC-Stella-Het-RRBS
GSM2068222 E13.5-Male-PGC-Stella-Null-RRBS
Relations
BioProject PRJNA312776
SRA SRP070645

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78149_RAW.tar 152.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap