|
| Status |
Public on Jun 07, 2016 |
| Title |
Ligation-Free Ribosome Profiling Reveals Cell Type-Specific Regulation of Protein Synthesis in the Brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Ribosome profiling has emerged as a powerful tool for genome-wide measurements of translation, but library construction requires multiple ligation steps and remains cumbersome relative to more conventional deep sequencing experiments. We report a new approach to ribosome profiling that does not require ligation. Library construction for ligation-free ribosome profiling can be completed in one day with as little as 1 ng of purified RNA footprints. We used ligation-free ribosome profiling to identify new patterns of cell type-specific translation in the brain and tested its ability to identify translational targets of mTOR signaling in the brain.
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| Overall design |
We report a strategy for ligation free cell-type specific ribosome profiling. Our strategy was utilized to explore cell-type specific expression in mouse brain.
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| Contributor(s) |
Sims PA, Hornstein N, Torres D, Sharma S, Tang G, Canoll P |
| Citation(s) |
27380875 |
| Submission date |
Feb 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter Sims |
| E-mail(s) |
pas2182@columbia.edu
|
| Organization name |
Columbia Medical Center
|
| Street address |
3960 Broadway blvd
|
| City |
New York |
| State/province |
NEW YORK |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA312799 |
| SRA |
SRP070655 |
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