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Series GSE7831 Query DataSets for GSE7831
Status Public on Dec 13, 2007
Title Expression data from immature pDC and pDC activated with CpG 1826 and influenza virus PR8
Organism Mus musculus
Experiment type Expression profiling by array
Summary CpG 1826 binds to Toll-like receptor (TLR)9, whereas influenza virus PR8 activates pDC via TLR7. Differential stimulation of pDCs is expected to result in unique activation mechanism(s) leading to a different phenotypically and functionally matured pDC
We used microarrays to detail the global programme of gene expression underlying the maturation process of pDC activated with CpG 1826 and influenza virus PR8. We identified a distinct expression profile of upregulated immunomediators.
Keywords: time course
 
Overall design Sorted pDCs were cultured for 1h and 4hs in medium control or with 5 µg/ml CpG 1826 or 300 HAU/ml purified influenza A/PR/8 virus. The first experiment (e1) included pDC in media and stimulated with CpG for 4h. In two other independent experimental batches (e2 and e3), we obtained samples of sorted pDC cultured in medium alone (med), and with CpG or PR8 (flu) for 1h and 4h. RNA extraction was performed using the RNeasy Kit (Qiagen) and hybridization on Affymetrix microarrays was performed using standard protocols. We sought to obtain homogeneous populations of pDCs at different time points under defined activation conditions in order to decipher the temporal resolution of expression profiles during the process of their maturation.
 
Contributor(s) Weninger W, Iparraguirre A, Tobias JW
Citation(s) 18029397
Submission date May 17, 2007
Last update date Feb 11, 2019
Contact name Amaya I Wolf
E-mail(s) aiwolf@wistar.org
Organization name Wistar Institute
Street address 3601 Spruce street
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM190131 pDC medium 1h, biological rep1
GSM190132 pDC medium 1h, biological rep2
GSM190133 pDC CpG 1h, biological rep1
Relations
BioProject PRJNA100069

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Supplementary file Size Download File type/resource
GSE7831_RAW.tar 51.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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