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Series GSE7838 Query DataSets for GSE7838
Status Public on Jul 01, 2007
Title Comprehensive Gene ExpressionProfiling of Peyer’s Patch M Cells, Villous M-like Cells, and Intestinal Epithelial Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Separate populations of M cells have been detected in the follicle-associated epithelium of Peyer’s patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that M cells are rare but M-like cells positive for lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM16-2-4 are induced by oral administration of cholera toxin in the duodenal villous epithelium. Here, we determined the gene expression of PP M cells, villous M-like cells, and intestinal epithelial cells (IECs) isolated by a novel approach using FACS. Specific expression of glycoprotein 2 (GP2) and MARCKS-like protein (MLP) by PP M cells and not villous M-like cells was confirmed by additional mRNA and protein analyses. Comprehensive gene profiling also suggested that villous M-like cells share traits of both PP M cells and IECs, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.
Keywords: Cell type comparison
Overall design Epithelial cells in duodenal Peyer's patches or villi of BALB/c mice treated with or without cholera toxin were dissociated and stained with NKM16-2-4 monoclonal antibody (mAb)-FITC, lectin UEA-1-PE, and anti-CD45 mAb-APC-Cy7. Naive PP M cells (NKM16-2-4+, UEA-1+, and CD45- cells), CT-induced villous M-like cells (NKM16-2-4+, UEA-1+, and CD45- cells), and naive intestinal epithelial cells (NKM16-2-4-, UEA-1-, and CD45- cells) were isolated by FACS. Affymetrix GeneChip Mouse Genome 430 2.0 Array was used in triplicate for each cell fraction.
Contributor(s) Terahara K, Igarashi O, Yoshida M, Nochi T, Kurokawa S, Takayama N, Yuki Y, Lowe AW, Kiyono H
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Submission date May 18, 2007
Last update date Feb 11, 2019
Contact name Kazutaka Terahara
Organization name The University of Tokyo
Department Institute of Medical Science
Street address 4-6-1 Shirokanedai
City Minato-ku
ZIP/Postal code 108-8639
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM190789 PP M CELL1
GSM190790 PP M CELL2
GSM190791 PP M CELL3
BioProject PRJNA99961

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7838_RAW.tar 32.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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