NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE784 Query DataSets for GSE784
Status Public on Oct 18, 2004
Title Transcriptional Profiling of the Transition from Normal Intestine to Adenoma in the APC(Min/+) Mouse
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional Profiling of the Transition from Normal Intestine to Adenoma in the APC(Min/+) Mouse.
Tissue was from male 91-days old APC(Min/+) mouse (an animal model for human colon cancer). RNA was purified using Trizol and labeled for hybridization to high density oligonucleotide Affymetrix MG_U74Av2 arrays, using manufacturer protocol. We measured the relative expression level of >12000 genes and ESTs.
-----------------------------------------
Samples used in analysis:
* GSM12501: Normal intestine diet #1 sample C1_0112 Dnmt+/- Min/+
* GSM12502: Tumor diet #1 sample T1_0112 Dnmt+/- Min/+
* GSM12503: Normal intestine diet #1 sample C2_0112 Dnmt+/+ Min/+
* GSM12504: Tumor diet #1 sample T2_0112 Dnmt+/+ Min/+
* GSM12505: Normal intestine diet #2 sample C1_003 Dnmt+/- Min/+
* GSM12506: Tumor diet #2 sample T1_003 Dnmt+/- Min/+
* GSM12507: Normal intestine diet #2 sample C2_003 Dnmt+/+ Min/+
* GSM12508: Tumor diet #2 sample T2_003 Dnmt+/+ Min/+
* GSM12509: Normal intestine diet #3 sample C1_005 Dnmt+/- Min/+
* GSM12510: Tumor diet #3 sample T1_005 Dnmt+/- Min/+
* GSM12511: Normal intestine diet #3 sample C2_005 Dnmt+/+ Min/+
* GSM12512: Tumor diet #3 sample T2_005 Dnmt+/+ Min/+
- - - - - - - - - - - - - - - - - - - - -
Comparisons were performed as described in Chen Z, Ge B, Hudson TJ and Rozen R. Gene Expression Patterns 1, 89-93, 2002. Comparing Normal intestine vs Adenoma.
- - - - - - - - - - - - - - - - - - - - -
This resulted in the identification of differentially expressed transcripts. Identified transcripts were clustered based on functional information which was publicly available at time of analysis, obtained through the NetAffx WEB portal (www.Affymetrix.com) and literature.
Keywords = Mouse
Keywords = Min
Keywords = normal
Keywords = intestine
Keywords = tumor
Keywords = tumour
Keywords = colon cancer
Keywords = gene expression
Keywords = beta-catenin
Keywords = high density oligonucleotide microarrays
Keywords = familial adenomatous polyposis
Keywords = FAP
Keywords = colorectal cancer
Keywords = Affymetrix MGU74Av2 GeneChip probe array
Keywords = mouse model
Keywords = APC
Keywords = adenomatous polyposis coli
Keywords: ordered
 
 
Contributor(s) Leclerc D, Deng L, Trasler J, Rozen R
Citation(s) 15486983
Submission date Oct 29, 2003
Last update date Feb 18, 2018
Contact name Daniel Leclerc
E-mail(s) daniel.leclerc@mail.mcgill.ca
Phone 514-412-4400 x23281
Fax 514-412-4331
Organization name Montreal Children's Hospital
Department Medical Genetics, Room 242
Lab Rima Rozen Laboratory
Street address 4060 Ste-Catherine West
City Montreal
State/province Quebec
ZIP/Postal code H3Z 2Z3
Country Canada
 
Platforms (1)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
Samples (12)
GSM12501 Normal intestine diet #1 sample C1_0112 Dnmt+/- Min/+
GSM12502 Tumor diet #1 sample T1_0112 Dnmt+/- Min/+
GSM12503 Normal intestine diet #1 sample C2_0112 Dnmt+/+ Min/+
Relations
BioProject PRJNA87749

Supplementary data header descriptions
category Functional classification
Gene Gene symbol
Acc._No GenBank accession number
Probe set Affymetrix Probe ID (MGU74Av2)
Gene Product Sequence description
Fold Change Fold change, normal intestine versus adenoma, based on 6 independent pairs +/- SE

Data table
category Gene Acc._No Probe set Gene Product Fold Change
Growth factors/related molecules (increased in tumor) Retnlb AA611740 93755_at resistin like beta 115 ± 73
Growth factors/related molecules (increased in tumor) RegIIIg D63362 96064_at regenerating gene III gamma 7 ± 1
Growth factors/related molecules (increased in tumor) Fgfrp U04204 100884_at fibroblast growth factor regulated protein 7 ± 1
Growth factors/related molecules (increased in tumor) Igfbp5 L12447 100566_at insulin-like growth factor binding protein 5 8 ± 1
Growth factors/related molecules (increased in tumor) Prss11 AW125478 96920_at protease, serine, 11/HTRA1 6 ± 2
Growth factors/related molecules (increased in tumor) Igfbp4 X76066 101571_g_at insulin-like growth factor binding protein 4 5 ± 1
Lipid metabolism (increased in tumor) Pla2g2a X74266 92735_at phospholipase A2, group IIA 20 ± 3
Lipid metabolism (increased in tumor) Pla2g12 AI845798 104343_f_at phospholipase A2, group XII-2 6 ± 1
Lipid metabolism (increased in tumor) Fabp6 U00938 99977_at fatty acid binding protein 6 (gastrotropin) 75 ± 13
Lipid metabolism (increased in tumor) Crbp1 X60367 104716_at retinol binding protein 1, cellular 5.1 ± 0.4
Lipid metabolism (increased in tumor) Scd2 M26270, AV327760 95758_at, 162077_f_at stearoyl-Coenzyme A desaturase 2 10 ± 2
ROS metabolism (increased in tumor) Gpx2 X91864 99810_at glutathione peroxidase 2 6 ± 1
ROS metabolism (increased in tumor) Gpx5 AV381732 161986_f_at glutathione peroxidase 5 13 ± 5
ROS metabolism (increased in tumor) Cyba AW046124 97013_f_at cytochrome b-245, alpha polypeptide 5 ± 1
Transcription factors (increased in tumor) Tgif X89749 101502_at TG3’ interacting factor 3.2 ± 0.1
Transcription factors (increased in tumor) Sox17 D49473 92996_at SRY-box containing gene 17 7 ± 1
Transcription factors (increased in tumor) Foxa1 U44752 92697_at forkhead box A1 6 ± 1
Transcription factors (increased in tumor) Tdag51 U44088 160829_at T-cell death associated gene 51 9 ± 1
Lytic enzymes/inhibitors (increased in tumor) Mcpt1 X68803, X68803, M57401 94728_f_at, 94729_r_at, 94735_s_at mast cell protease 1 69 ± 23
Lytic enzymes/inhibitors (increased in tumor) Cma2 M68899 95806_f_at chymase 2, mast cells 17 ± 4

Total number of rows: 90

Table truncated, full table size 9 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap