NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE7846 Query DataSets for GSE7846
Status Public on Oct 31, 2007
Title Differentially expressed genes in HEECs of eutopic endometrium of patients with endometriosis compared with control
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The pathogenesis of endometriosis may result from aberrant angiogenesis that occurs in eutopic endometrium with retrograde menstruation. The difference in gene expression profile between human endometrial endothelial cells (HEECs) from eutopic endometria of patients with and without endometriosis would be determinant that affects the occurrence of endometriosis. To explore this kind of difference, we performed in vitro culture and identified their endothelial origin, as well as observed growth features of HEECs from the two different origins. Finally we identified the difference in gene expression profile when combined suppression subtractive hybridization(SSH) with genechip and confirmed the results by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The HEECs derived from endometriosis exhibited potent survival ability in vitro compared to that from non-endometriosis. We found that gremlin and fibronectin genes were up-regulated in HEECs derived from eutopic endometrium of patients with endometriosis when compared with that from patients without endometriosis. Our study implies that enhanced angiogenic capacity of eutopic HEECs may be an independent determinant in endometriotic aberrant angiogenesis in addition to the interaction of exfoliated endometrium and peritoneal environment elements such as activated macrophages and soluble cytokines.
Keywords: human endometrial endothelial cells (HEECs),endometriosis,differentially expressed genes
 
Overall design We analyzed 5 arrays for HEECs derived from eutopic endometrium of patients with endometriosis and 5 arrays for HEECs derived from that of patients without endometriosis
 
Citation(s) 17956924
Submission date May 18, 2007
Last update date Mar 25, 2019
Contact name Guihua Sha
E-mail(s) shaguihua1970@yahoo.com.cn
Organization name Peking Union Medical College Hospital
Street address 1 Wangfujing, Shuaifuyuan
City BeiJing
ZIP/Postal code 100730
Country China
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (10)
GSM190395 eutopic endometrium from patients with endometriosis, biological rep1
GSM190396 eutopic endometrium from patients with endometriosis, biological rep2
GSM190397 eutopic endometrium from patients with endometriosis, biological rep3
Relations
BioProject PRJNA99977

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7846_RAW.tar 43.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap