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Series GSE7852 Query DataSets for GSE7852
Status Public on Jul 27, 2009
Title Fat Treg cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Comparisons of global gene-expression profiles revealed a greater distinction between CD4+ Treg cells and CD4+ conventional (Tconv) T cells residing in abdominal (epidydimal) fat versus in more standard locations such as the spleen, thymus and LN.
Keywords: Cell population comparison
 
Overall design All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 3-5 x 104 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
 
Contributor(s) Feuerer M, Benoist C, Mathis D
Citation(s) 19633656
Submission date May 18, 2007
Last update date Feb 11, 2019
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM176892 TregThy, rep1
GSM176893 TconvThy, rep1
GSM176897 TregLN, rep1
Relations
BioProject PRJNA99989

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7852_RAW.tar 66.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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