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Status |
Public on Jun 27, 2007 |
Title |
Gene expression comparison of mouse ES cells and EpiSCs |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The application of human embryonic stem (ES) cells in medicine and biology has an inherent reliance on understanding the starting cell population. Human ES cells differ from mouse ES cells and the specific embryonic origin of both cell types is unclear. Previous work suggested that mouse ES cells could only be obtained from the embryo before implantation in the uterus1–5. Here we show that cell lines can be derived from the epiblast, a tissue of the postimplantation embryo that generates the embryo proper. These cells, which we refer to as EpiSCs (post-implantation epiblastderived ES cells), express transcription factors known to regulate pluripotency, maintain their genomic integrity, and robustly differentiate into the major somatic cell types as well as primordial germ cells. The EpiSC lines are distinct from mouse ES cells in their epigenetic state and the signals controlling their differentiation. Furthermore, EpiSC and human ES cells share patterns of gene expression and signalling responses that normally function in the epiblast. These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development. *Note: EpiSCs were previously referred to as post-ES cells Keywords: cell type comparison
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Overall design |
3 biological replicates of each cell type (EpiSC and mouse ES) were compared.
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Contributor(s) |
Tesar PJ, Chenoweth JG, McKay R |
Citation(s) |
17597760 |
Submission date |
May 21, 2007 |
Last update date |
May 10, 2018 |
Contact name |
Paul Tesar |
E-mail(s) |
paul.tesar@case.edu
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Organization name |
Case Western Reserve University
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Department |
Genetics & Genome Sciences
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Street address |
10900 Euclid Ave.
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City |
Cleveland |
State/province |
Ohio |
ZIP/Postal code |
44106 |
Country |
USA |
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Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE7902 |
Novel cell lines from mouse epiblast share defining features with human embryonic stem cells |
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Relations |
BioProject |
PRJNA105501 |