|
| Status |
Public on Oct 31, 2003 |
| Title |
Corticosteroid responsive genes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by SAGE
|
| Summary |
Three groups of Wistar rats with different manipulated levels of corticosterone were used to identify corticosteroid-responsive genes in hippocampus:
1. rats were adrenalectomised (ADX)
2. In addition to ADX these rats were implanted with a subcutaneous pellet containing 20 mg corticosterone and 80% cholesterol (ADX + low CORT)
3. In addition to ADX and implantation of a corticosterone pellet, these rats received a subcutaneous injection with corticosterone (1 mg/kg bodyweight) three hours prior to decapitation ((ADX + low CORT) + acute high CORT).
All 3 groups of rats were decapitated three days after ADX and their hippocampus was isolated. Hippocampal RNA from 6 rats per group was pooled and used as input material for the SAGE libraries.
Keywords: parallel sample
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| Contributor(s) |
Datson N, van der Perk J, de Kloet E, Vreugdenhil E |
| Citation(s) |
11556892 |
| Submission date |
Oct 30, 2003 |
| Last update date |
Mar 02, 2012 |
| Contact name |
Nicole Datson |
| E-mail(s) |
datson_n@lacdr.leidenuniv.nl
|
| Phone |
(+31)71-5276222
|
| Organization name |
Leiden University
|
| Department |
Medical Pharmacology
|
| Street address |
|
| City |
Leiden |
| ZIP/Postal code |
2333 CC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL23 |
SAGE:10:NlaIII:Rattus norvegicus |
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| Samples (3) |
| GSM12542 |
rat hippocampus: adrenalectomised rat (ADX) |
| GSM12543 |
rat hipocampus: ADX + low CORT |
| GSM12544 |
rat hippocampus: (ADX + low CORT)+ acute high CORT |
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| Relations |
| BioProject |
PRJNA87755 |
