|
| Status |
Public on May 08, 2016 |
| Title |
A long noncoding RNA lincRNA-EPS acts as a transcriptional brake to restrain inflammation |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
LincRNA-EPS is a long noncoding RNA that is expressed in macrophages and downregulated upon exposure to diverse microbial products. Analysis of macrophages from lincRNA-EPS-deficient mice revealed a specific role for this lincRNA in restraining immune response gene (IRG) expression. Mechanistically, lincRNA-EPS associates with chromatin at regulatory regions of IRGs to repress their transcription. To gain insight into the molecular function of lincRNA-EPS on chromatin, we generated ATAC-Seq libraries from wildtype and lincRNA-EPS-deficient macrophages stimulated with LPS.
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| |
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| Overall design |
We examined chromatin structure using ATAC-seq in wild-type and lincRNA-EPS knock-out macrophages stimulated with LPS
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| Contributor(s) |
Atianand M, Shen Y, Satpathy A, Chang H, Fitzgerald K |
| Citation(s) |
27315481 |
| Submission date |
Mar 03, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Howard Chang |
| Organization name |
Stanford University
|
| Street address |
269 Campus Dr. Stanford, CA
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA314247 |
| SRA |
SRP071137 |
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