|
| Status |
Public on May 25, 2007 |
| Title |
S1P mediates key targets associated with survival, proliferation and pluripotency in human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human embryonic stem cells (hESCs) replicate by the process of self-renewal, whilst maintaining their pluripotency. Understanding the pathways involved in the regulation of this self-renewal process will assist in developing fully-defined conditions for the proliferation of hESCS required for therapeutic applications. We previously demonstrated a role for Sphingosine-1-phosphate (S1P) in the survival and proliferation of hESCs. The present study investigates further key signalling pathways and the downstream targets of S1P.
Microarrays were used to examine changes in gene expression invoked by treatment of human embryonic stem cells grown upon different matrices
Keywords: Karyotypically normal human Esc's
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| |
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| Overall design |
Human embryonic stem cells (Sheff 4) were grown upon MEFs. One group were treated with S1P. Each group consisted of three replicates which were hybridised to Human U133 plus 2 Affymetrix GeneChips
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| |
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| Contributor(s) |
Inniss K, Avery S, Shepherd J, Heath P, Moore H |
| Citation(s) |
18393631 |
| Submission date |
May 24, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Paul Roy Heath |
| E-mail(s) |
p.heath@sheffield.ac.uk
|
| Phone |
+44 114 2222254
|
| Organization name |
University of Sheffield
|
| Department |
Academic Neurology Unit
|
| Lab |
Heath
|
| Street address |
SITraN, 385a Glossop Road
|
| City |
Sheffield |
| State/province |
S. Yorks |
| ZIP/Postal code |
S10 2HQ |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
|
| GSM194310 |
Sheff 4 hesc S1P treated replicate1 |
| GSM194311 |
Sheff 4 hesc S1P treated replicate 2 |
| GSM194312 |
Sheff 4 hesc S1P treated replicate 3 |
| GSM194313 |
Sheff 4 hesc grown upon Matrigel replicate 2 |
| GSM194314 |
Sheff 4 hesc grown upon Matrigel replicate 3 |
|
| Relations |
| BioProject |
PRJNA99943 |
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