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| Status |
Public on Dec 21, 2007 |
| Title |
Transcriptional profiling of inductive prostatic mesenchyme |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by SAGE
|
| Summary |
Background: The mesenchymal compartment plays a key role in organogenesis and cells within the mesenchyme/stroma are a source of potent molecules that control epithelia during development and tumourigenesis. We have used Serial Analysis of Gene Expression (SAGE) to profile a key subset of prostatic mesenchyme that regulates prostate development and is enriched for growth-regulatory molecules.
Results: SAGE libraries were made of prostatic inductive mesenchyme (VMP) and the complete prostatic rudiment (including inductive mesenchyme, epithelium and smooth muscle; VSU). By comparing these two SAGE libraries we generated a list of 219 transcripts that were enriched or specific to inductive mesenchyme and which may act as mesenchymal regulators of organogenesis and tumourigenesis.
Conclusions: The use of a precisely defined subset of cells, and a back-comparison approach, allowed us to identify rare mRNAs that might be overlooked using other approaches.
Keywords: SAGE, gene profiling
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| Overall design |
Two SAGE libraries were prepared and compared to each other. The VMP forms a subset of the VSU.
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| Contributor(s) |
Orr B, Thomson AA |
| Citation(s) |
17922897 |
| Submission date |
May 25, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Axel Thomson |
| E-mail(s) |
a.thomson@hrsu.mrc.ac.uk
|
| Phone |
+44 131 242 6221
|
| Fax |
+44 131 242 6231
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| Organization name |
MRC Human Reproductive Sciences Unit
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| Street address |
47 Little France Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4TJ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL5330 |
SAGE:17:NlaIII:Rattus norvegicus |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA99949 |
