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| Status |
Public on Apr 19, 2016 |
| Title |
Gene expression data from long term hematopoietic stem cells (LT-HSC, Lin-Sca-1+c-kit+CD34-CD135-) isolated from control, MxCre Jak2VF/+ and MxCre Jak2VF/+ EZH2-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inactivating EZH2 mutations have been associated with myelofibrosis (MF). Moreover, EZH2 mutations co-exist with the JAK2V617F mutation in a significant cases of MF. To determine the effects of concomitant loss of EZH2 and JAK2V617F mutation in hematopoiesis, we generated Ezh2-deficient Jak2V617F-expressing mice. To gain insights into the mechanisms by which Ezh2 deficiency promotes the development of MF in Jak2V617F knock-in mice, we performed microarray gene expression analysis on sorted LT-HSC from control, MxCre;Jak2VF/+ and MxCre;Jak2VF/+ EZH2-/- mice.
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| Overall design |
Microarray analyses were performed on LT-HSC sorted from control, MxCre Jak2VF/+ and MxCre Jak2VF/+ EZH2-/- mice BM cells at 12 weeks after pI-pC injections. Three biological replicates for each genotype, i.e., a total of nine samples were utilized for microarray analyses.
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| Contributor(s) |
Yang Y, Mohi G |
| Citation(s) |
27081096 |
| Submission date |
Mar 14, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Golam Mohi |
| E-mail(s) |
mohim@upstate.edu
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| Organization name |
SUNY Upstate Medical University
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| Department |
Pharmacology
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| Street address |
766 Irving Avenue
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| City |
Syracuse |
| State/province |
New York |
| ZIP/Postal code |
13210 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM2087917 |
MxCre Jak2VF/+ LT-HSC, biological rep1 |
| GSM2087918 |
MxCre Jak2VF/+ LT-HSC, biological rep2 |
| GSM2087919 |
MxCre Jak2VF/+ LT-HSC, biological rep3 |
| GSM2087920 |
MxCre Jak2VF/+ EZH2-/- LT-HSC, biological rep1 |
| GSM2087921 |
MxCre Jak2VF/+ EZH2-/- LT-HSC, biological rep2 |
| GSM2087922 |
MxCre Jak2VF/+ EZH2-/- LT-HSC, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE79472 |
Loss of Ezh2 cooperates with Jak2V617F in the development of myelofibrosis in a mouse model of myeloproliferative neoplasm |
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| Relations |
| BioProject |
PRJNA315136 |
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