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| Status |
Public on Mar 15, 2017 |
| Title |
The impact of decitabine treatment in MDS-induced Tet2KD/KDEzh2Δ/Δ LSK HSPCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To evaluate the impact of DNA demethylating agents on our mouse MDS model, we chose 5-aza-2’-deoxycytidine, decitabine (DAC), one of the DNA demethylating agents, which is incorporated into DNA but not RNA and has 10-fold more potency in DNA demethylation than 5-azacitidine. We transplanted Tet2KD/KDEzh2Δ/Δ MDS cells into lethally irradiated secondary recipients and treated them with DAC (low dose DAC at 0.25mg/kg, 3 times a week, intraperitoneal injection), then purified LSK HSPCs and evaluated the expression profiles.
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| Overall design |
We purified Tet2KD/KDEzh2Δ/Δ -MDS Lineage-Sca-1+c-Kit+ (LSK) HSPCs from secondary recipients 10 weeks post-transplantation and 7 weeks after DAC or PBS treatment and subjected them to microarray analysis.
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| Contributor(s) |
Hasegawa N, Oshima M, Iwama A |
| Citation missing |
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| Submission date |
Mar 14, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Motohiko Oshima |
| E-mail(s) |
oshima@faculty.chiba-u.jp
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| Phone |
+81432262187
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| Organization name |
Chiba University
|
| Street address |
1-8-1 Inohana
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| City |
Chiba |
| State/province |
State... |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (2) |
| GSM2087974 |
BM-LSK_Tet2KD/KDEzh2Δ/Δ -MDS_BMT-2nd_DAC |
| GSM2087975 |
BM-LSK_Tet2KD/KDEzh2Δ/Δ -MDS_BMT-2nd_PBS |
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| Relations |
| BioProject |
PRJNA315154 |
