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| Status |
Public on Mar 15, 2017 |
| Title |
Epithelial-to-mesenchymal transition defines feedback activation of receptor tyrosine kinase signaling induced by MEK inhibition in KRAS mutant lung cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To determine the difference of gene expression profile in epithelial and mesenchymal KRAS mutant lung cancers, epithelial NCI-H358 cells were treated with TGFβ1 (4 ng/mL) or PBS for 14 days in order to induce epithelial to mesenchymal transition (EMT). Gene expression was determined in NCI-H358 cells before and after EMT induction. In addition, in order to investigate the effect of a MEK inhibitor trametinib on gene expression, mesenchymal NCI-H1792 cells were treated with 50 nM trametinib for 48 hours. Gene expression of H1792 cells for pre- and post-trametinib treatement was determined.
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| Overall design |
H358 was induced EMT by treated with TGF-beta. H1792 were treated with trametinib for 48 hours. Two independent experiments were performed
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| Contributor(s) |
Ebi H, Kitai H, Tomida S |
| Citation(s) |
27154822 |
| Submission date |
Mar 15, 2016 |
| Last update date |
Oct 03, 2019 |
| Contact name |
Hiromichi Ebi |
| E-mail(s) |
hebi@staff.kanazawa-u.ac.jp
|
| Organization name |
Kanazawa University
|
| Department |
Institute for Frontier Science Initiative and Cancer Research Institute
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| Lab |
Ebi laboratory
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| Street address |
13-1 Takaramachi
|
| City |
Kanazawa |
| State/province |
Ishikawa |
| ZIP/Postal code |
920-0934 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA315250 |
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