|
| Status |
Public on Mar 16, 2016 |
| Title |
Gene expression in colorectal liver metastasis tissues from EPA-treated patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Eicosapentaenoic acid in its free fatty acid form (EPA-FFA), 2g daily, is safe and well-tolerated in patients undergoing liver resection surgery for colorectal liver metastasis.Oral EPA incorporates into colorectal liver metastasis tissue. EPA-FFA treatment is associated with reduced vascularity of liver metastases in ω-3 PUFA-naïve patients. Preoperative (median 30 days) EPA-FFA treatment may have prolonged benefit on postoperative overall and disease-free survival.
We used whole genome expression array to study whether systemic CCL2 level changes were linked to a specific tumour gene expression profile in colorectal liver metastasis patients treated with EPA-FFA.
|
| |
|
| Overall design |
15 tumour RNA samples from colorectal liver metastasis patients treated with EPA-FFA during the EMT study (ClinicalTrials.gov NCT01070355) were extracted from formalin-fixed paraffin-wax embedded tissue blocks. The RNA samples were used for whole genome expression microarray experiments. We then performed a differential gene expression analysis to compare the tumour expression profile of patients with increased or decreased plasma CCL2 levels after intervention.
|
| |
|
| Contributor(s) |
Volpato M, Perry SL, Marston G, Ingram N, Cockbain AJ, Burghel H, Mann J, Lowes D, Wilson E, Droop A, Randerson-Moor J, Coletta PL, Hull MA |
| Citation(s) |
24470281 |
| Submission date |
Mar 15, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Milene Volpato |
| E-mail(s) |
medmvo@leeds.ac.uk
|
| Organization name |
University of Leeds
|
| Department |
LIRM
|
| Street address |
St James University Hospital
|
| City |
LEEDS |
| ZIP/Postal code |
LS9 7TF |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA315304 |
Less...
More...