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| Status |
Public on Oct 21, 2016 |
| Title |
AP-1 and TEAD4 co-operatively promote the vascular program during hemangioblast specification [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Embryonic blood cell development occurs via well-defined developmental stages which are recapitulated in vitro by differentiation of embryonic stem cells. This process is tightly regulated by the interaction of tissue- specific and ubiquitous transcription factors with the chromatin landscape in response to outside signals. We previously identified binding motifs for the commonly expressed AP-1 transcription factor family in open chromatin regions specific for early stages of blood specification and thus aimed to study the role of AP-1 for hemangioblast differentiation. Here we show that FOS and JUN together bind to and activate a core set of vascular genes in the hemogenic endothelium and that upon global inhibition of AP-1 by expression of a dominant negative FOS peptide the balance between endothelial and hematopoietic fate is shifted towards blood. Moreover, we demonstrate that in the hemogenic endothelium AP-1 is required for de novo binding of TEAD4, a transcription factor connected to Hippo signaling, to vascular genes. Notably, after the endothelial-to-hematopoietic transition TEAD4 binding is no longer persisting. These findings provide novel mechanistic insights into vascular and hematopoietic development.
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| Overall design |
Examination of transcription binding patterns, gene expression patterns in developing hemogenic and hematopoietic cells with and without AP-1, high-resolution DNaseI accessibility in hemogenic endothelium and hematopoietic progenitors
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| Contributor(s) |
Obier N, Cauchy P, Assi SA, Gilmour J, Lie-A-Ling M, Hoogenkamp M, Lichtinger M, Noailles L, Cockerill P, Lacaud G, Kouskoff V, Bonifer C |
| Citation(s) |
27802171 |
| Submission date |
Mar 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Pierre Daniel Cauchy |
| E-mail(s) |
cauchy@ie-freiburg.mpg.de
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| Phone |
+49 (0)761 270-77576
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| Organization name |
University Medical Center Freiburg
|
| Department |
Zentrum für Translationale Zellforschung
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| Lab |
AG Onco-Immunology
|
| Street address |
Breisacher Str. 115
|
| City |
Freiburg |
| State/province |
Baden-Württemberg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
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| Platforms (4)
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| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE79323 |
AP-1 and TEAD4 co-operatively promote the vascular program during hemangioblast specification |
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| Relations |
| BioProject |
PRJNA315457 |
| SRA |
SRP071904 |
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