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| Status |
Public on Dec 31, 2016 |
| Title |
Transcriptomic signatures of villous cytotrophoblast and syncytiotrophoblast in term human placenta. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
During pregnancy, the placenta ensures multiple functions, which are directly involved in the initiation, fetal growth and outcome of gestation. The placental tissue involved in maternal-fetal exchanges and in synthesis of pregnancy hormones is the mononucleated villous cytotrophoblast (VCT) which aggregates and fuses to form and renew the syncytiotrophoblast (ST). Knowledge of the gene expression pattern specific to this endocrine and exchanges tissue of human placenta is of major importance to understand functions of this heterogeneous and complex tissue. Therefore, we undertook a global analysis of the gene expression profiles of primary cultured-VCT (n=6) and in vitro-differentiated-ST (n=5) in comparison with whole term placental tissue from which mononucleated VCT were isolated. A total of 880 differentially expressed genes (DEG) were observed between VCT/ST compared to whole placenta, and a total of 37 and 137 genes were significantly up and down-regulated, respectively, in VCT compared to ST. The 37 VCT-genes were involved in cellular processes (assembly, organization, and maintenance), whereas the 137 ST-genes were associated with lipid metabolism and cell morphology. In silico, all networks were linked to 3 transcriptional regulators (PPARγ, RARα and NR2F1) which are known to be essential for trophoblast differentiation. Furthermore, a subset of DEG were validated by RT-qPCR or by immunohistochemistry. To conclude, recognition of these pathways is fundamental to increase our understanding of the molecular basis of human trophoblast differentiation. The present study provides for the first time a gene expression signature of the VCT and ST compared to their originated term human placental tissue.
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| Overall design |
One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion), and 3 µg of amplified RNA (aRNA) was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences). To compare the gene expression pattern obtained from each cultured cells (VCT or ST) with the one obtained from corresponding total placental extracts, amplified RNA from cultured cells was labeled with Cy5, while the aRNA from placental tissue was labeled with Cy3. A total of six (VCT) or five (ST) individual cDNA microarrays were performed in this condition.
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| Contributor(s) |
Rouault C, Clément K, Guesnon M, Henegar C, Charles M, Heude B, Evain-Brion D, Degrelle SA, Fournier T |
| Citation(s) |
27452442 |
| Submission date |
Mar 17, 2016 |
| Last update date |
Apr 01, 2017 |
| Contact name |
Severine Aude Degrelle |
| E-mail(s) |
severine.degrelle@inserm.fr
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| Organization name |
INSERM UMRS-1139
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| Street address |
4 avenue de l'Observatoire
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| City |
PARIS |
| ZIP/Postal code |
75006 |
| Country |
France |
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| Platforms (1) |
| GPL21609 |
Stanford Functional Genomics Facility SHDZ |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA315528 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79333_RAW.tar |
42.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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