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| Status |
Public on Mar 22, 2016 |
| Title |
Transcriptional profile of C. elegans fed E. coli (OP50) and B. subtilis (PY79) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used RNA-seq to assay gene expression changes over time in response to OP50 and PY79
To understand the molecular processes underlying aging, we screened modENCODE ChIP-seq data to identify transcription factors that bind to age-regulated genes in C. elegans. The most significant hit was the GATA transcription factor encoded by elt-2, which is responsible for inducing expression of intestinal genes during embryogenesis. Expression of ELT-2 decreases during aging, beginning in middle age. We identified genes regulated by ELT-2 in the intestine during embryogenesis, and then showed that these developmental genes markedly decrease in expression as worms grow old. Overexpression of elt-2 extends lifespan and slows the rate of gene expression changes that occur during normal aging. Thus, our results identify the developmental regulator ELT-2 as a major driver of normal aging in C. elegans.
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| Overall design |
Whole-worm mRNA was sequenced from E. coli- and B.subtilis-fed worms. For each condidtion, one replicate was sequenced at Day 4 and Day 13
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| Contributor(s) |
Mann FG, Kim SK |
| Citation(s) |
27070429 |
| Submission date |
Mar 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Stuart Kim |
| E-mail(s) |
stuartkm@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Developmental Biology
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| Street address |
279 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA315807 |
| SRA |
SRP072091 |
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