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Series GSE7957 Query DataSets for GSE7957
Status Public on Oct 08, 2007
Title Expression data from Pseudomonas aeruginosa exposed airway epithelium from C57Bl6 and MMP-7 and MMP-10 deficient mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Airway epithelium is the initial point of host-pathogen interaction in Pseudomonas aeruginosa infection, an important pathogen in cystic fibrosis and nosocomial pneumonia. We used global gene expression analysis to determine airway epithelial transcriptional responses dependent on matrilysin (MMP-7) and stromelysin-2 (MMP-10), two matrix metalloproteinases induced by acute P. aeruginosa pulmonary infection. Extraction of Differential Gene Expression (EDGE) analysis of gene expression changes in P. aeruginosa infected organotypic tracheal epithelial cell cultures from wildtype, Mmp7-/-, and Mmp10-/- mice identified 2,089 matrilysin-dependent and 1,628 stromelysin-2-dependent genes that were differentially expressed. Key node network analysis showed that these MMPs controlled distinct gene expression programs involved in proliferation, cell death, immune responses, and signal transduction, among other host defense processes. Our results demonstrate discrete roles for these MMPs in regulating epithelial responses to pseudomonas infection and show that a global genomics strategy can be used to assess MMP function.
Keywords: time course
 
Overall design C57Bl6, Mmp7-/- and Mmp10-/- mouse epithelium at an organotypic air liquid interface culture was exposed to Pseudomonas aeruginosa for 1 and 24 h. RNA was collected from these samples as well as uninfected 0 h and assessed for expression changes using Affymetrix Mouse 430 2.0 Arrays. Triplicate samples were processed for each genotype at each time point.
 
Contributor(s) Kassim SY, Gharib SA, Mecham BH, Birkland TP, Parks WC, McGuire JK
Citation(s) 17923522
Submission date May 30, 2007
Last update date Feb 11, 2019
Contact name Sean Y Kassim
E-mail(s) seanyk@u.washington.edu
Phone 206-897-1307
Organization name University of Washington
Department Medicine, PCCM
Lab Center for Lung Biology
Street address 815 Mercer St
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (25)
GSM196062 Wtepi_infect_0h_rep1
GSM196063 Wtepi_infect_0h_rep2
GSM196064 Wtepi_infect_0h_rep3
Relations
BioProject PRJNA100703

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7957_RAW.tar 78.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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