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| Status |
Public on Sep 01, 2016 |
| Title |
High conservation in transcriptomic and proteomic response of white sturgeon to equipotent concentrations of 2,3,7,8-TCDD, PCB 77, and benzo[a]pyrene |
| Organism |
Acipenser transmontanus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Adverse effects associated with exposure to dioxin-like compounds (DLCs) are mediated primarily through activation of the aryl hydrocarbon receptor (AHR). However, little is known about the cascades of events that link activation of the AHR to apical adverse effects. Therefore, this study used high-throughput, next-generation molecular tools to investigate similarities and differences in whole transcriptome and whole proteome responses to equipotent concentrations of three agonists of the AHR, 2,3,7,8-TCDD, PCB 77, and benzo[a]pyrene, in livers of a non-model fish, the white sturgeon (Acipenser transmontanus). A total of 926 and 658 unique transcripts were up- and down-regulated, respectively, by one or more of the three chemicals. Of the transcripts shared by responses to all three chemicals, 85% of up-regulated transcripts and 75% of down-regulated transcripts had the same magnitude of response. A total of 290 and 110 unique proteins were up- and down-regulated, respectively, by one or more of the three chemicals. Of the proteins shared by responses to all three chemicals, 70% of up-regulated proteins and 48% of down-regulated proteins had the same magnitude of response. Among treatments there was 68% similarity between the global transcriptome and global proteome. Pathway analysis revealed that perturbed physiological processes were indistinguishable between equipotent concentrations of the three chemicals. The results of this study contribute towards more completely describing adverse outcome pathways associated with activation of the AHR.
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| Overall design |
Compared transcriptomic as well as proteomic responses to 3 different agonists of the aryl hydrocarbon receptor (AHR) at equipotent doses.
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| Contributor(s) |
Doering JA, Tang S, Peng H, Eisner B, Sun J, Giesy J, Wiseman S, Hecker M, Patterson S, Zee J, Beitel S |
| Citation(s) |
27070345 |
| Submission date |
Mar 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jon A Doering |
| E-mail(s) |
jad929@mail.usask.ca
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| Organization name |
University of Saskatchewan
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| Department |
Toxicology Centre
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| Street address |
44 Campus Drive
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| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7N 5B3 |
| Country |
Canada |
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| Platforms (2) |
| GPL21657 |
Illumina MiSeq (Acipenser transmontanus) |
| GPL21658 |
Illumina HiSeq 2000 (Acipenser transmontanus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA316466 |
| SRA |
SRP072366 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE79624_RAW.tar |
2.8 Mb |
(http)(custom) |
TAR (of CSV, TXT) |
| GSE79624_Reference_Transcriptome.txt.gz |
21.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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