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Status |
Public on Mar 26, 2016 |
Title |
Overexpression of LMO2 causes aberrant human T-cell development in vivo by three potentially distinct cellular mechanisms |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
LMO2 overexpressing transgenic mouse models suggest an accumulation of immature T-cell progenitors in the thymus as main pre-leukemic event. The effects of LMO2 overexpression on human T-cell development in vivo, however, are unknown. Here we report studies of a humanized mouse model transplanted with LMO2 transduced human hematopoietic stem and progenitor cells. The effects of LMO2 overexpression were confined to the T-cell lineage although initially multipotent cells were transduced. Three effects of LMO2 on human T-cell development were observed: 1) a block at the DN/ISP stage, 2) an accumulation of CD4+CD8+ double positive CD3- cells and 3) an altered CD8/CD4 ratio with enhanced peripheral T lymphocytes
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Overall design |
Single stranded cDNA was synthesized from 1000 pg total RNA using the Ovation Pico WTA System V2 Module (NuGEN Technologies Inc, Leek, The Netherlands) in combination with the Encore Biotin Module (NuGEN Technologies Inc) according to the instructions of the manufacturer. Biotin labeling and fragmentation was performed and fragmented cDNA was hybridized onto Affymetrix Gene Atlas human U219 arrays.
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Contributor(s) |
Wiekmeijer A, Pike-Overzet K, Brugman MH, Van Eggermond MC, Cordes M, De Haas EF, LI Y, Oole E, Van Ijcken WF, Egeler RM, Meijerink JP, Staal FJ |
Citation(s) |
27302866 |
Submission date |
Mar 25, 2016 |
Last update date |
Mar 21, 2019 |
Contact name |
Martijn Cordes |
E-mail(s) |
martijn.cordes@gmail.com
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Organization name |
Leiden University Medical Centre
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Department |
Immunology
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Lab |
Staal
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Street address |
Albinusdreef 2
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City |
Leiden |
State/province |
Zuid-Holland |
ZIP/Postal code |
2333 ZA |
Country |
Netherlands |
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Platforms (1) |
GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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Samples (4)
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Relations |
BioProject |
PRJNA316470 |