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Series GSE79649 Query DataSets for GSE79649
Status Public on Mar 29, 2016
Title RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells (RIP)
Organism Mus musculus
Experiment type Other
Summary We performed an integrated analysis of RNA and proteins at the transition between naïve ES cells and cells primed to differentiate. During this transition, mRNAs coding for chromatin regulators were specifically released from translational inhibition mediated by RNA-Induced Silencing Complex (RISC). This suggests that, prior to differentiation, the propensity of ES cells to change their epigenetic status is hampered by RNA interference. The expression of these chromatin regulators was reinstated following acute inactivation of RISC, and it correlated with loss of stemness markers and activation of early cell differentiation markers in treated ES cells.
 
Overall design Cytoplasmic RNA-protein complexes from mouse embryonic stem cells at different stages of neural in vitro differentiation were immunoprecipitated with anti-Argonaute antibody and analyzed by microarray hybridization
 
Contributor(s) Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, Rocchiccioli S, D'Onofrio M, Cremisi F
Citation(s) 27154007
Submission date Mar 28, 2016
Last update date Feb 02, 2018
Contact name Luca Pandolfini
E-mail(s) luca.pandolfini@iit.it
Organization name Istituto Italiano di Tecnologia - I
Department Center for Human Technologies
Street address Via Enrico Melen 83, Building B
City Genova
State/province Genova
ZIP/Postal code 16152
Country Italy
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (8)
GSM2100467 E14Tg2A_0DIV_AgoIP
GSM2100468 E14Tg2A_0DIV_totRNA
GSM2100469 E14Tg2A_2DIV_AgoIP
This SubSeries is part of SuperSeries:
GSE79655 RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells
Relations
BioProject PRJNA316609

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79649_RAW.tar 97.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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