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| Status |
Public on Mar 29, 2016 |
| Title |
RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells (RNAi) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We performed an integrated analysis of RNA and proteins at the transition between naïve ES cells and cells primed to differentiate. During this transition, mRNAs coding for chromatin regulators were specifically released from translational inhibition mediated by RNA-Induced Silencing Complex (RISC). This suggests that, prior to differentiation, the propensity of ES cells to change their epigenetic status is hampered by RNA interference. The expression of these chromatin regulators was reinstated following acute inactivation of RISC, and it correlated with loss of stemness markers and activation of early cell differentiation markers in treated ES cells.
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| Overall design |
We assayed the impact of the functional inhibition of chromatin regulators on cell priming inhibiting chromatin remodeling activities by either pharmacological or genetic interference.
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| Contributor(s) |
Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, Rocchiccioli S, D'Onofrio M, Cremisi F |
| Citation(s) |
27154007 |
| Submission date |
Mar 28, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Luca Pandolfini |
| E-mail(s) |
luca.pandolfini@iit.it
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| Organization name |
Istituto Italiano di Tecnologia - I
|
| Department |
Center for Human Technologies
|
| Street address |
Via Enrico Melen 83, Building B
|
| City |
Genova |
| State/province |
Genova |
| ZIP/Postal code |
16152 |
| Country |
Italy |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE79655 |
RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA316607 |
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