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| Status |
Public on Jun 03, 2008 |
| Title |
CD34 gene silencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to investigate the role of CD34 antigen in haematopoietic commitment, we silenced the CD34 gene expression in CD34+ stem/progenitor cells using a siRNA approach.
Keywords: treatment comparison
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| Overall design |
To maximize siRNA transfection efficiency, we utilized the NucleofectorTM technology (Amaxa). CD34+ cells were transfected with a mixture of 4 siRNAs targeting CD34 mRNA and with a non-targeting siRNA as a negative control. The expression level of CD34 antigen on control cells (MOCK and negative control treated cells) and CD34siRNA treated cells was assessed by immunofluorescence analysis at 24 and 48h post-nucleofection.
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| Contributor(s) |
Salati S, Zini R, Bianchi E, Testa A, Mavilio F, Manfredini R, Ferrari S |
| Citation(s) |
18192237 |
| Submission date |
Jun 04, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Rossella Manfredini |
| E-mail(s) |
manfredini.rossella@unimore.it
|
| Phone |
+390592058065
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| Organization name |
Centre for Regenerative Medicine
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| Department |
Life Sciences
|
| Street address |
Via Gottardi 100
|
| City |
Modena |
| ZIP/Postal code |
41100 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE8040 |
CD34 antigen role in haematopoietic commitment |
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| Relations |
| BioProject |
PRJNA105387 |
