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Series GSE80139 Query DataSets for GSE80139
Status Public on Jun 15, 2016
Title Notch signaling promotes differentiation of mouse mucosal mast cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Intestinal mucosal mast cells are critically involved in the development of food-induced allergic disorders. However, factors that induce differentiation of mucosal mast cells in the intestinal mucosa are largely unknown.
To identify factors involved in mucosal mast cell differentiation, we compared the gene expression profiles between mucosal mast cells isolated from the small intestine and bone marrow-derived mast cells cultured in the presence of TGF-β or Notch ligand.
 
Overall design Mucosal mast cells were isolated from the small intestine of naïve BALB/c mice by flow cytometry. Bone marrow-derived mast cells (BMMCs) were generated by culturing BALB/c bone marrow cells with murine interleukin-3 and stem cell factor for 3-4 weeks, and then cells were cultured for 6 days in the presence or absence of TGF-β or Delta-like 1 (Dll1), which is a Notch ligand. Total RNAs extracted from these cells were processed and hybridized to Affymetrix GeneChips.
 
Contributor(s) Nakano N, Honjo A
Citation(s) 27555456
Submission date Apr 11, 2016
Last update date Feb 21, 2018
Contact name Nobuhiro Nakano
E-mail(s) nbnakano@juntendo.ac.jp
Organization name Juntendo University
Department Graduate School of Medicine
Lab Atopy (Allergy) Research Center
Street address 2-1-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8421
Country Japan
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (4)
GSM2113442 BMMCs, rep1
GSM2113443 BMMCs cultured with TGF-β, rep1
GSM2113444 BMMCs cultured with Notch ligand Dll1, rep1
Relations
BioProject PRJNA318022

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80139_RAW.tar 33.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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