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| Status |
Public on Sep 01, 2017 |
| Title |
Gemone-wide changes of epigenetic mark H3K27 trimethylation (H3K27me3) and AR in prostate cancer cells upon the treatment of EZH2 inhibitors [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Our initial data showed different responses of abl and DU145 cells to EZH2 inhibitors, therefore we detected the chromatin binding signals of H3K27me3 in both cell lines in the presence of the compounds. We found that genome-wide changes in H3K27me3 levels were not associated with the inhibitor-mediated transcriptional programs or biological effects. Since the gene expression profiles upon the compound treatment indicated an important role of AR signaling in mediating the inhibitory action of the drugs in abl cells, we them mapped AR chromatin binding patterns in the presence or absence of EZH2 inhibitors. It was interesting to detect a dramatic decline in AR binding intesities at regulatory regions of the regulated target genes.
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| Overall design |
Detection of the changes in chromatin binding intesities/signals of H3K27me3 and AR upon EZH2 inhibitors in prostate cancer cells
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| |
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| Contributor(s) |
Xu K, Chen C |
| Citation(s) |
35031563 |
| Submission date |
Apr 13, 2016 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Kexin Xu |
| E-mail(s) |
kxuthscsa@gmail.com
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Molecular Medicine
|
| Street address |
7703 Floyd Curl, MC 8257
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE80240 |
Distinct changes in transcriptional profiles and epigenetic patterns mediated by EZH2 inhibitors in sensitive and insensitive prostate cancer cells |
|
| Relations |
| BioProject |
PRJNA318381 |
| SRA |
SRP073246 |
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