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Series GSE8034 Query DataSets for GSE8034
Status Public on May 29, 2008
Title Prospective isolation of functionally distinct radial glial subtypes - lineage and transcriptome analysis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of
these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia.
Overall design Comparison of radial glial subtypes
Contributor(s) Pinto L, Mader MT, Irmler M, Gentilini M, Santoni F, Bulfone A, Malatesta P, Beckers J, Götz M, Drechsel D, Blum R, Stahl R
Citation(s) 18372191
Submission date Jun 06, 2007
Last update date Feb 11, 2019
Contact name Johannes Beckers
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (17)
GSM198357 E18 cortex GFP+ Prominin+ sample S3
GSM198361 E18 cortex GFP+ Prominin+ cells, sample S2
GSM198362 E18 cortex GFP+ Prominin+ cells, sample S4
BioProject PRJNA100847

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Supplementary file Size Download File type/resource
GSE8034_RAW.tar 58.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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