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Series GSE8039 Query DataSets for GSE8039
Status Public on Oct 30, 2007
Title Gene Expression in Transgenic Mice Expressing CD30L on T Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary CD30L and CD30 are cell-surface glycoproteins in the TNF and TNFR superfamilies, respectively. Their expression is limited to immune cells and is tightly regulated. Cell surface expression of CD30 is restricted to subpopulations of activated T and B cells. CD30L is expressed primarily on activated T cells and subpopulations of B cells. The significance of CD30/CD30L interactions in immune regulation is not fully understood. Reported activities of CD30/CD30L in immune responses imply roles in regulation of secondary memory and antibody responses. Depending on the experimental system, both positive and negative regulation of immunoglobulin class switching and antibody production have been reported. Additionally, the biological activity of CD30/CD30L in animals has been difficult to assess due to the restricted and tightly regulated expression of this receptor-ligand pair. We generated transgenic mice with constitutive T cell specific overexpression of CD30L as a tool to help unravel the consequences of CD30/CD30L interactions in vivo. CD30L transgenic mice displayed a phenotype and responses to antigen challenge supporting a role for CD30/CD30L in promoting immunoglobulin class switching and antibody production. CD30L transgenic mice had increased numbers of germinal centers, elevated class-switched immunoglobulin isotypes, increased germinal center B cells and plasma cells, upregulation of genes indicative of B-cell activity, and exaggerated antibody responses to immune challenge. Interestingly, despite the heightened B-cell activity in CD30L transgenic mice, CD30L overexpression on T cells did not result in overt autoimmunity. Our results demonstrate that overexpression of CD30L on T cells promotes T cell-dependent B cell responses characterized by secondary antibody responses.
Keywords: Transgenic vs. wild-type
 
Overall design Spleen, thymus, mesenteric lymph nodes and peripheral lymph nodes where samples from both CD30L transgenic and non-transgenic littermates (4 mice per group.)
 
Contributor(s) Willis CR
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Submission date Jun 06, 2007
Last update date Feb 11, 2019
Contact name John Gosink
E-mail(s) gosinkj@amgen.com
Phone 206 265-8217
Organization name Amgen
Street address 1201 Amgen Court West
City Seattle
State/province WA
ZIP/Postal code 98119
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (32)
GSM198409 T1 CD2-CD30L peripheral lymph node
GSM198410 T2 CD2-CD30L peripheral lymph node
GSM198411 C1 peripheral lymph node
Relations
BioProject PRJNA100855

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8039_RAW.tar 200.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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