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| Status |
Public on Dec 01, 2017 |
| Title |
Whole genome landscapes of major melanoma subtypes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cutaneous, acral and mucosal subtypes of melanoma were evaluated by whole-genome sequencing, revealing genes affected by novel recurrent mutations to the promoter (TERT, DPH3, OXNAD1, RPL13A, RALY, RPL18A, AP2A1), 5’-UTR (HNRNPUL1, CCDC77, PES1), and 3’-UTR (DYNAP, CHIT1, FUT9, CCDC141, CDH9, PTPRT) regions. TERT promoter mutations had the highest frequency of any mutation, but neither they nor ATRX mutations, associated with the alternative telomere lengthening mechanism, were correlated with greater telomere length. Genomic landscapes largely reflected ultraviolet radiation mutagenesis in cutaneous melanoma and provided novel insights into melanoma pathogenesis. In contrast, acral and mucosal melanomas exhibited predominantly structural changes, and mutation signatures of unknown aetiology not previously identified in melanoma. The majority of melanomas had potentially actionable mutations, most of which were in components of the mitogen-activated protein kinase and phosphoinositol kinase pathways.
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| Overall design |
Expression arrays from 65 of the 183 tumours analysed were used to determine gene expression levels of genes with recurrent promoter and 3' and 5' UTR mutations. A total of 32 samples is available at GSE54467; the remaining 33 samples are submitted here. The 27 primary melanoma samples (AJCC stage II) were assayed using the HumanHT-12 v4 Expression BeadChip (Illumina® Inc., San Diego, CA, USA; Catalog IDs: BD-103-0204, BD-103-0604). The remaining 6 metastatic (AJCC stage IV) samples were assayed using the HumanWG-6 v3 Expression BeadChip ((Illumina® Inc., San Diego, CA, USA; Catalog IDs: BD-101-0203, BD-101-0603). NEQC normalisation (default parameters) was separately applied to each of the AJCC stage II and IV datasets (http://0-nar-oxfordjournals-org.brum.beds.ac.uk/content/38/22/e204). Probes for which there were no samples with a detection p-value of less than 0.01 were removed.
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| Contributor(s) |
Hayward NK, Wilmott JS, Waddell N, Johansson PA, Field MA, Nones K, Patch A, Kakavand H, Alexandrov LB, Burke H, Jakrot V, Kazakoff S, Holmes O, Leonard C, Wood S, Xu Q, Waddell N, Tembe V, Pupo GM, De Paoli-Iseppi R, Vilain RE, Shang P, Schramm S, Pritchard A, Dutton-Regester K, Fitzgerald A, Shang CA, Grimmond SM, Yang JY, Stretch JR, Kefford RF, Hersey P, Long GV, Cebon J, Shackleton M, Spillane AJ, Saw RP, Pearson JV, Thompson JF, Scolyer RA, Mann GJ |
| Citation(s) |
28467829 |
| Submission date |
Apr 19, 2016 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Graham J. Mann |
| E-mail(s) |
graham.mann@sydney.edu.au
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| Organization name |
Melanoma Institute Australia
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| Street address |
Poche Centre, 40 Rocklands Road
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| City |
North Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2060 |
| Country |
Australia |
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| Platforms (2) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (33)
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| Relations |
| BioProject |
PRJNA318904 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE80435_RAW.tar |
32.4 Mb |
(http)(custom) |
TAR |
| GSE80435_non_normalized_stage_2.txt.gz |
7.9 Mb |
(ftp)(http) |
TXT |
| GSE80435_non_normalized_stage_4.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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