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| Status |
Public on Apr 22, 2017 |
| Title |
ppmax2 response to gr24-Strigopath/Physcomitrella patens |
| Organism |
Physcomitrium patens |
| Experiment type |
Expression profiling by array
|
| Summary |
4plex_physco_2014-05 - ppmax2 response to gr24 - How does the Ppmax2 moss mutant respond to Strigolactone (GR24)? - Two moss genotypes are used: WT and the Ppmax2 mutant. Moss tissues are fragmented, then plated on medium (Petri dish with cellophane disks) and cultivated for 3 weeks. Moss tissues are then transfered for 6 hours on acetone-containing medium (control treatment, for WT and Ppmax2) or GR24 (1 microM, in acetone)-containing medium (for Ppmax2). After 6 hours, the moss tissues are collected, quickly forzen in liquid nitrogen. RNA are isolated using the Quiagen RNeasy Plant mini kit (including a RNase-free DNase treatment on column). Two similar experiments (T1 and T2) have been led.
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| Overall design |
4 dye-swap - genotype comparaison,normal vs transgenic comparaison,treated vs untreated comparison
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| Web link |
http://urgv.evry.inra.fr/cgi-bin/projects/CATdb/consult_expce.pl?experiment_id=583
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| Contributor(s) |
Bonnard G, Pateyron S, Rameau C, Balzergue S, Martin-Magniette M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 22, 2016 |
| Last update date |
Apr 23, 2017 |
| Contact name |
Stéphanie Pateyron |
| E-mail(s) |
pateyron@evry.inra.fr
|
| Organization name |
IPS2_Institute of Plant Sciences Paris-Saclay
|
| Lab |
Transcriptomic Plateforme POPS
|
| Street address |
Rue de Noetzlin _ Batiment 630
|
| City |
Orsay |
| ZIP/Postal code |
91405 |
| Country |
France |
| |
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| Platforms (1) |
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| Samples (8)
|
| GSM2131069 |
256685310003_b1 - WT+ac_1 vs Ppmax2+ac_1 |
| GSM2131070 |
256685310003_b2 - Ppmax2+ac_1 vs WT+ac_1 |
| GSM2131071 |
256685310003_b3 - WT+ac_2 vs Ppmax2+ac_2 |
| GSM2131072 |
256685310003_b4 - Ppmax2+ac_2 vs WT+ac_2 |
| GSM2131073 |
256685310004_b1 - Ppmax2+ac_1 vs Ppmax2+GR24_1 |
| GSM2131074 |
256685310004_b2 - Ppmax2+GR24_1 vs Ppmax2+ac_1 |
| GSM2131075 |
256685310004_b3 - Ppmax2+ac_2 vs Ppmax2+GR24_2 |
| GSM2131076 |
256685310004_b4 - Ppmax2+GR24_2 vs Ppmax2+ac_2 |
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| Relations |
| BioProject |
PRJNA319344 |
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