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Series GSE80618 Query DataSets for GSE80618
Status Public on Apr 26, 2016
Title Loss of renal tubular PGC-1α exacerbates diet-induced renal steatosis and age-related urinary sodium excretion in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The kidney has a high energy demand and is dependent on oxidative metabolism for ATP production. Accordingly, the kidney is rich in mitochondria, and mitochondrial dysfunction is a common denominator for several renal diseases. While the mitochondrial master regulator peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) is highly expressed in kidney, its role in renal physiology is so far unclear. Here we show that PGC-1α is a central transcriptional regulator of mitochondrial metabolic pathways in the kidney. Moreover we demonstrate that mice with an inducible nephron-specific inactivation of PGC-1α in the kidney display elevated urinary sodium excretion, exacerbated renal steatosis during metabolic stress but normal blood pressure regulation. Overall, PGC-1α seems largely dispensable for basal renal physiology. However, the central role of PGC-1α in renal mitochondrial biogenesis indicates that activation of PGC-1α in the context of renal disorders could be a valid therapeutic strategy to ameliorate renal mitochondrial dysfunction.
 
Overall design We used microarrays to detect changes in gene expression in whole kidney from mice. Nephron-specific inducible PGC-1alpha knockout (NiPKO) or control mice (CTRL) were fed either a chow diet or a high fat diet (HFD 60 kcal% fat, D12492) for 20 weeks. We used 4 biological replicates for each condition. The samples were: CWT – chow fed CTRL mice; CKO – chow fed NiPKO mice; HWT – HFD fed CTRL mice; HKO – HFD fed NiPKO mice.
 
Contributor(s) Svensson K, Cardel B, Handschin C
Citation(s) 27463191
Submission date Apr 25, 2016
Last update date Feb 21, 2018
Contact name Christoph Handschin
Organization name Biozentrum, University of Basel
Street address Klingelbergstrasse 50/70
City Basel
ZIP/Postal code 4056
Country Switzerland
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (16)
GSM2131790 Chow CTRL biological rep. 1
GSM2131791 Chow CTRL biological rep. 2
GSM2131792 Chow CTRL biological rep. 3
Relations
BioProject PRJNA319496

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80618_RAW.tar 145.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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