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| Status |
Public on May 26, 2016 |
| Title |
Human Calu-3 cell transcriptome response to wild-type pandemic H1N1 (A/California/04/2009), natural isolate [mRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type pandemic H1N1 (A/California/04/2009), natural isolate.
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| Overall design |
Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Cells were infected with an MOI of 3. After infection, wash and replace with 2 ml of DMEM-F12 + .3% BSA and 1 ug/ml TPCK-trypsin. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12, 24, 36, and 48 hr post-infection.
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| Contributor(s) |
Walters K, Stratton K, Thompson A, Waters KM, Eisfeld AJ, Kawaoka Y |
| Citation(s) |
34051754 |
| Submission date |
Apr 26, 2016 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
|
| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (57)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA319728 |
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