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Series GSE8091 Query DataSets for GSE8091
Status Public on May 29, 2008
Title Transcriptome and proteome analysis of early embryonic mouse brain development
Organism Mus musculus
Experiment type Expression profiling by array
Summary Embryonic mouse brain development involves a sequential differentiation of multipotent progenitor cells into neurons and glia. Using microarrays and large 2-D electrophoresis, we investigated the transcriptome and proteome of mouse brains at embryonic days 9.5, 11.5 and 13.5. During this developmental period, neural progenitor cells shift from proliferation to neuronal differentiation. As expected, we detected numerous expression changes between the time points investigated but interestingly, the rate of alteration was about 10% to 13% of all proteins and mRNAs during every two days of development. Furthermore, up- and downregulation was balanced. This was confirmed for two additional stages of development, embryonic day 16 and 18. We hypothesize that during embryonic development, the rate of protein expression alteration is rather constant due to a limitation of cellular resources such as energy, space and free water. The similar complexity found at the transcriptome and proteome level at all stages suggests, that changes in relative concentration of gene products rather than an increased number of gene products dominate throughout cellular differentiation. We found that metabolism and cell cycle related gene products were downregulated in expression when precursor cells switched from proliferation to neuronal differentiation (day 9.5 to 11.5), whereas neuron specific gene products were upregulated. A detailed analysis revealed their implication in differentiation related processes such as rearrangement of the actin cytoskeleton as well as Notch and Wnt signaling pathways.
Keywords: time course, development, transcriptome, proteome
Overall design 3 embryonic stages:
E9.5: 6 biological replicates
E11.5: 4 biological replicates
E13.5: 6 biological replicates
Contributor(s) Hartl D, Irmler M, Römer I, Mader MT, Mao L, Zabel C, Hrabé de Angelis M, Beckers J, Klose J
Citation(s) 18283662
Submission date Jun 12, 2007
Last update date Feb 11, 2019
Contact name Johannes Beckers
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM200695 E9.5 embryo head sample13
GSM200696 E9.5 embryo head sample 15
GSM200697 E9.5 embryo head sample 16
BioProject PRJNA100939

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Supplementary file Size Download File type/resource
GSE8091_RAW.tar 58.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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