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Series GSE81100 Query DataSets for GSE81100
Status Public on Jan 14, 2017
Title Aging induces de novo rhythmic expression of oxidative stress-responsive genes
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Disruption of the circadian clock, which directs rhythmic expression of numerous
genes, accelerates aging. To inquire how the circadian system protects organisms
during aging, we compared circadian transcriptomes in heads of young and old
Drosophila melanogaster. These data revealed a class of genes that adopt de novo
rhythmicity during aging, termed "late life cyclers" (LLCs). We show that application of exogenous oxidative stress in young flies mimics aging by inducing robust, rhythmic LLC upregulation in a light- and CLOCK-dependent fashion. Additionally, we identified age-onset rhythmicity in some primary piRNA transcripts that overlap antisense transposable elements. To share our data, we developed a database for public viewing of graphical RNA-seq results for coding and non-coding RNAs in young and old flies. Taken together, our results suggest that aging organisms recruit the circadian system to launch a temporally coordinated defense to cope with their increasingly stressful cellular environments.
 
Overall design RNA-Seq was collected around the clock every 4 hours for young (5 day) and old (55 day) Drosophila melanogaster heads.
 
Contributor(s) Hendrix DA, Kuintzle RC
Citation(s) 28221375, 36561348
Submission date May 04, 2016
Last update date Jan 11, 2023
Contact name David Anthony Hendrix
E-mail(s) david.hendrix@oregonstate.edu
Phone (541) 737-6224
Organization name Oregon State University
Department Biochemistry and Biophysics/EECS
Street address 2011 Ag & Life Sciences Bldg
City Corvallis
State/province OR
ZIP/Postal code 97331
Country USA
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (24)
GSM2143625 dme_young_ZT0_RNASeq_r1
GSM2143626 dme_young_ZT0_RNASeq_r2
GSM2143627 dme_young_ZT4_RNASeq_r1
Relations
BioProject PRJNA320747
SRA SRP074424

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81100_old_ARSER_output.txt.gz 1.7 Mb (ftp)(http) TXT
GSE81100_old_ZT0_tophat_dm6_gene_exp.diff.gz 589.7 Kb (ftp)(http) DIFF
GSE81100_old_ZT12_tophat_dm6_gene_exp.diff.gz 596.1 Kb (ftp)(http) DIFF
GSE81100_old_ZT16_tophat_dm6_gene_exp.diff.gz 595.2 Kb (ftp)(http) DIFF
GSE81100_old_ZT20_tophat_dm6_gene_exp.diff.gz 596.7 Kb (ftp)(http) DIFF
GSE81100_old_ZT4_tophat_dm6_gene_exp.diff.gz 596.3 Kb (ftp)(http) DIFF
GSE81100_old_ZT8_tophat_dm6_gene_exp.diff.gz 599.3 Kb (ftp)(http) DIFF
GSE81100_old_vs_GSE81100_youngdm6_gene_exp.diff.gz 596.0 Kb (ftp)(http) DIFF
GSE81100_youngARSER_output.txt.gz 1.7 Mb (ftp)(http) TXT
GSE81100_youngZT0_tophat_dm6_gene_exp.diff.gz 594.0 Kb (ftp)(http) DIFF
GSE81100_youngZT12_tophat_dm6_gene_exp.diff.gz 587.9 Kb (ftp)(http) DIFF
GSE81100_youngZT16_tophat_dm6_gene_exp.diff.gz 591.8 Kb (ftp)(http) DIFF
GSE81100_youngZT20_tophat_dm6_gene_exp.diff.gz 595.1 Kb (ftp)(http) DIFF
GSE81100_youngZT4_tophat_dm6_gene_exp.diff.gz 598.1 Kb (ftp)(http) DIFF
GSE81100_youngZT8_tophat_dm6_gene_exp.diff.gz 594.1 Kb (ftp)(http) DIFF
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Raw data are available in SRA
Processed data are available on Series record

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