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| Status |
Public on Apr 21, 2017 |
| Title |
Expression data of GFP-neg and GFP-high cells isolated from mouse SCLC tumors using a GFP reporter for Hes1 expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Small cell lung cancer (SCLC) tumors harbor two subsets of cells that are defined by low (or undetectable) or high expression of Hes1, which is a common downstream target of the Notch signaling pathway.
To better understand the role of the Notch pathway in SCLC tumor heterogeneity, we first isolated cells from these two populations using a GFP-reporter allele for Hes1 expression. We then used microarrays to identify the gene expression differences between these two groups.
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| Overall design |
SCLC tumors were induced by Cre-administration in an Rb/p53/p130 conditional triple knockout mouse model for SCLC that also carried a GFP-reporter allele for Hes1 expression. GFP-negative and GFP-high cells were FACS sorted from pooled tumors 5-6 months after tumor induction. RNA was then extracted and hybridized to Affymetrix GeneChip® Mouse Gene 2.0 ST array.
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| Contributor(s) |
Lim JS, Sage J |
| Citation(s) |
28489825 |
| Submission date |
May 05, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Julien Sage |
| Organization name |
Stanford
|
| Department |
Pediatrics and Genetics
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| Lab |
Sage
|
| Street address |
265 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5457 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2144104 |
(Hes1)GFP-negative cells, biological rep 1 |
| GSM2144105 |
(Hes1)GFP-negative cells, biological rep 2 |
| GSM2144106 |
(Hes1)GFP-negative cells, biological rep 3 |
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| Relations |
| BioProject |
PRJNA320772 |
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