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Series GSE81242 Query DataSets for GSE81242
Status Public on Oct 01, 2016
Title Gene expression of skeletal muscles in response to overexpression of Notch1 intracellular domain (N1ICD)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analysis of gene expression profile of Tibialis anterior (TA) skeletal muscle tissues with Notch1 intracellular domain (N1ICD) overexpression. Skeletal myogenesis involves sequential activation, proliferation, self-renewal/differentiation and fusion of myogenic stem cells (satellite cells). Notch signaling is known to be essential for the maintenance of satellite cells, but its function in late-stage myogenesis, i.e. post-differentiation myocytes and post-fusion myotubes, is unknown. Here we use muscle creatine kinase (MCK)-Cre to induce N1ICD expression in multinucleated myotubes. We found that myotube-specific Notch1 activation improved muscle regeneration and exercise performance of mdx mice, a model of Duchenne Muscular Dystrophy (DMD). Agilent microarray was performed to compare gene expression in mdx control and N1ICD-overexpressing mdx muscles. The results may provide mechanistic insights into how Notch1 activation in myotubes modulate muscle function.
 
Overall design Two-condition experiment, mdx muscle vs. N1ICD-mdx muscle. Biological replicates: 3 mdx TA muscles and 3 N1ICD-mdx TA muscles.
Please note that experiments were performed technically as dual channel (eg, Cy3-labeled-mdx and Cy5-labeled-N1ICD samples hybridized to the same array) but the results were processed as though they are single channel (Cy3 and Cy5 signals are calculated).
 
Contributor(s) Bi P, Yue F, Wirbisky S, Freeman JL, Kuang S
Citation(s) 27644105
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AR060652 Notch Regulation of Stem Cell Fate in Muscle PURDUE UNIVERSITY Kuang
Submission date May 09, 2016
Last update date Jul 19, 2017
Contact name Shihuan Kuang
E-mail(s) Skuang@purdue.edu
Organization name Purdue University
Street address 901 W State St
City West Lafayette
State/province IN
ZIP/Postal code 47907
Country USA
 
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM2147062 mdx TA replicate 1
GSM2147065 mdx TA replicate 2
GSM2147067 mdx TA replicate 3
Relations
BioProject PRJNA321037

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81242_RAW.tar 7.9 Mb (http)(custom) TAR
GSE81242_SG12494259_252800520725_S001_GE2_1105_Oct12_1_1.txt.gz 4.5 Mb (ftp)(http) TXT
GSE81242_SG12494259_252800520725_S001_GE2_1105_Oct12_1_2.txt.gz 5.9 Mb (ftp)(http) TXT
GSE81242_SG12494259_252800520725_S001_GE2_1105_Oct12_1_3.txt.gz 5.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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