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Status |
Public on Oct 01, 2016 |
Title |
Gene expression of skeletal muscles in response to overexpression of Notch1 intracellular domain (N1ICD) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Analysis of gene expression profile of Tibialis anterior (TA) skeletal muscle tissues with Notch1 intracellular domain (N1ICD) overexpression. Skeletal myogenesis involves sequential activation, proliferation, self-renewal/differentiation and fusion of myogenic stem cells (satellite cells). Notch signaling is known to be essential for the maintenance of satellite cells, but its function in late-stage myogenesis, i.e. post-differentiation myocytes and post-fusion myotubes, is unknown. Here we use muscle creatine kinase (MCK)-Cre to induce N1ICD expression in multinucleated myotubes. We found that myotube-specific Notch1 activation improved muscle regeneration and exercise performance of mdx mice, a model of Duchenne Muscular Dystrophy (DMD). Agilent microarray was performed to compare gene expression in mdx control and N1ICD-overexpressing mdx muscles. The results may provide mechanistic insights into how Notch1 activation in myotubes modulate muscle function.
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Overall design |
Two-condition experiment, mdx muscle vs. N1ICD-mdx muscle. Biological replicates: 3 mdx TA muscles and 3 N1ICD-mdx TA muscles. Please note that experiments were performed technically as dual channel (eg, Cy3-labeled-mdx and Cy5-labeled-N1ICD samples hybridized to the same array) but the results were processed as though they are single channel (Cy3 and Cy5 signals are calculated).
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Contributor(s) |
Bi P, Yue F, Wirbisky S, Freeman JL, Kuang S |
Citation(s) |
27644105 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 AR060652 |
Notch Regulation of Stem Cell Fate in Muscle |
PURDUE UNIVERSITY |
Kuang |
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Submission date |
May 09, 2016 |
Last update date |
Jul 19, 2017 |
Contact name |
Shihuan Kuang |
E-mail(s) |
Skuang@purdue.edu
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Organization name |
Purdue University
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Street address |
901 W State St
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City |
West Lafayette |
State/province |
IN |
ZIP/Postal code |
47907 |
Country |
USA |
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Platforms (1) |
GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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Samples (6)
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Relations |
BioProject |
PRJNA321037 |