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| Status |
Public on Dec 14, 2016 |
| Title |
HOX13 activity reprograms cis-regulatory modules during digit development (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The combinatorial expression of the Hox genes along the body axes, referred to as the HOX code, is a major determinant of cell fate and plays a prevailing role in generating the animal body plan. In developing limb buds, the paralogous group 13 genes of the HoxA and HoxD clusters are essential for patterning the distal-most limb structures, the digits. Inactivation of HOXA13 and HOXD13 transcription factors (HOX13) leads to complete digit agenesis in mice, but how HOX13 regulate transcriptional outcomes and confer identity to the distal-most limb cells has remained elusive. Here we performed genome-wide profiling of HOX13 by chromatin immunoprecipitation and analyzed the transcriptome and chromatin state of wild type early and late-distal limb buds, as well as Hoxa13-/-;Hoxd13-/- compound mutant limb buds. Our results show that inactivation of HOX13 impairs the activation and repression of putative cis-regulatory modules specific to the late-distal limb cells. Loss of HOX13 also disrupts the specific, spatial patterning of gene expression along the proximal-distal axis of the developing limb buds. These results show that proper termination of the early limb transcriptional program and activation of the late-distal limb program are coordinated by the dual action of HOX13 on cis-regulatory modules.
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| |
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| Overall design |
Totla mRNAs from dissected distal parts of e11.5 forelimb, of wild-type as well as Hoxa13-/-;Hoxd13-/- mice
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| Contributor(s) |
Sheth R, Barozzi I, Langlais D, Osterwalder M, Nemec S, Stadler HS, Visel A, Drouin J, Kmita M |
| Citation(s) |
27974206, 29490982, 34548488 |
| Submission date |
May 11, 2016 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Rushikesh Sheth |
| Organization name |
IRCM
|
| Department |
Genetics and Development
|
| Street address |
110 avenue des pins ouest
|
| City |
montreal |
| ZIP/Postal code |
H2W 1R7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE81358 |
HOX13 activity reprograms cis-regulatory modules during digit development |
|
| Relations |
| BioProject |
PRJNA321344 |
| SRA |
SRP074864 |
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