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Series GSE81474 Query DataSets for GSE81474
Status Public on May 07, 2017
Title Directed differentiation of human embryonic stem cells to corneal endothelial cell-like cells: A transcriptomic analysis
Organism Homo sapiens
Experiment type Third-party reanalysis
Expression profiling by high throughput sequencing
Summary The corneal endothelium is composed of a monolayer of corneal endothelial cells (CECs), which is essential for maintaining corneal transparency. Human embryonic stem cells (hESCs) hold the promise of providing an abundant donor source for generating CEC cells for cell replacement therapies. Here we demonstrate that CEC-like cells can be efficiently derived from human ESCs. In addition, we performed global gene expression profiling of stem-cell-derived CEC cells, incorporating with adult CEC cells, fetal CEC cells and other human tissue type data. Our data indicate that hESC-derived CEC-like cells closely resemble human fetal CEC cells.
Overall design We analyzed a total of 44 samples.
6 samples were sequenced by our group. High-throughput RNA-seq data from human ES cell-derived CEC-like cells samples (n=4) and cultured adult CEC samples (n=2) using Illumina HiSeq 2000.
38 samples are publicly available in GEO.
The entire set of processed data is linked below as supplementary file 44_samples_RPKM.txt.
Contributor(s) Yuan S, Hu G, Song Q, An Q, Fan G
Citation(s) 27523468
Submission date May 16, 2016
Last update date May 15, 2019
Contact name Qin An
Organization name ucla
Department Department of Human Genetics
Lab Guoping Fan Lab
Street address Room 6554 Gonda Building, UCLA
City los angeles
State/province CALIFORNIA
ZIP/Postal code 90024
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM2153009 aCEC66_RNAseq
GSM2153010 aCEC67_RNAseq
GSM2153011 CEC_like#1_RNAseq
Reanalysis of GSM1369182
Reanalysis of GSM1369183
Reanalysis of GSM1369184
Reanalysis of GSM1556368
Reanalysis of GSM1556369
Reanalysis of GSM1556370
Reanalysis of GSM2071044
Reanalysis of GSM1830134
Reanalysis of GSM1830135
Reanalysis of GSM752705
Reanalysis of GSM752706
Reanalysis of GSM742928
Reanalysis of GSM931711
Reanalysis of GSM931712
Reanalysis of GSM931713
Reanalysis of GSM752691
Reanalysis of GSM752692
Reanalysis of GSM752693
Reanalysis of GSM1333749
Reanalysis of GSM1333751
Reanalysis of GSM1333752
Reanalysis of GSM742925
Reanalysis of GSM742941
Reanalysis of GSM2055786
Reanalysis of GSM742937
Reanalysis of GSM742921
Reanalysis of GSM1808042
Reanalysis of GSM752707
Reanalysis of GSM752708
Reanalysis of GSM742950
Reanalysis of GSM742946
Reanalysis of GSM742930
Reanalysis of GSM1970433
Reanalysis of GSM1020212
Reanalysis of GSM1020213
Reanalysis of GSM1020214
Reanalysis of GSM1020215
Reanalysis of GSM1020216
BioProject PRJNA324132
SRA SRP075990

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Supplementary file Size Download File type/resource
GSE81474_44_samples_RPKM.txt.gz 2.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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