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Series GSE81581 Query DataSets for GSE81581
Status Public on Jun 12, 2017
Title Genomic characterization of liver metastases from colorectal cancer patients [miRNA-3]
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Metastatic dissemination is the most frequent cause of death sporadic colorectal cancer (sCRC) patients. The genomic abnormalities which are potentially characteristic of such advanced stages of the disease are complex and so far, they have been poorly described and only partially understood. We evaluate the molecular heterogeneity of sCRC tumors based on simultaneous assessment of the overall GEP of both coding mRNA and non-coding RNA genes in primary sCRC tumor samples from 23 consecutive patients and their paired liver metastases. Liver metastases from the sCRC patients analyzed, systematically showed deregulated transcripts of those genes identified as also deregulated in their paired primary colorectal carcinomas. However, some transcripts were found to be specifically deregulated in liver metastases while expressed at normal levels in their primary tumors, suggesting an increased genomic instability of neoplastic cells from metastatic versus primary tumor samples. Of note, once tumoral samples were normalized vs. non-tumoral colorectal tissue, only three mRNAS (i.e.: DEFB1, COL12A and PTGER3) and one miRNA (i.e.: miR-572) emerged as significantly deregulated in the liver metastases vs. the primary tumor. Canonical pathways found to be specifically deregulated in liver metastatic samples included multiple genes related with intercellular adhesion and the metastatic processes (e.g., IGF1R, PIK3CA, PTEN and EGFR), endocytosis (e.g., the PDGFRA, SMAD2, ERBB3, PML and FGFR2), and cell cycle (e.g., SMAD2, CCND2, E2F5 and MYC). Our results also highlight the activation of genes associated with the TGFβ signaling pathway, which thereby emerge as candidate genes to play an important role in CRC tumor metastasis.
 
Overall design Tissue specimens from 23 sporadic colorectal adenocarcinomas and 19 liver metastases (n=42 samples) were obtained from 23 patients with metastatic lesions susceptible of being resected (16 males and 7 females; median age of 66 years, ranging from 48 to 80 years) after informed consent had been given by each subject. The studied cases corresponded to 23 consecutive metastatic colorectal cancer patients.
 
Contributor(s) Sayagues JM, Corchete L, Gutierrez ML, del Mar Abad M, Bengoechea O, Fermiñan E, Anduaga MF, Iglesias M, Esteban C, Angoso M, Alcazar JA, Garcia J, Orfão A, Muñoz-Bellvis L
Citation(s) 27662660
Submission date May 18, 2016
Last update date Jul 27, 2018
Contact name Jose María Sayagués
E-mail(s) ppmari@usal.es
Organization name Cancer Research Center (IBMCC-CSIC/USAL)
Department Department of Medicine
Lab Cytometry Service-NUCLEUS
Street address Campus Miguel de Unamuno
City Salamanca
State/province Salamanca
ZIP/Postal code 37007
Country Spain
 
Platforms (1)
GPL16384 [miRNA-3] Affymetrix Multispecies miRNA-3 Array
Samples (51)
GSM2157575 Non-tumoral_colorectal_tissue_6391 (miRNA-3)
GSM2157576 Non-tumoral_colorectal_tissue_F249 (miRNA-3)
GSM2157577 Non-tumoral_colorectal_tissue_F251 (miRNA-3)
This SubSeries is part of SuperSeries:
GSE81582 Genomic characterization of liver metastases from colorectal cancer patients
Relations
BioProject PRJNA322040

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Supplementary file Size Download File type/resource
GSE81581_RAW.tar 68.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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