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Status |
Public on May 24, 2016 |
Title |
Effects of blocking IL-17A on lung gene expression after ozone exposure in obese (db/db) mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Obese mice have an augmented response to Ozone exposure (2 ppm for 3 hours and studied 24 hours later), including increase airway hyperresponsiveness and BAL inflammation when compared to WT ozone exposed mice. When IL-17A is block with systemic injected of IL-17A antibodies there is a decrease of response in the obese mice. To determine the mechanism of action, RNA was extracted from the lungs of IL-17A and Isotype injected ozone exposed obese mice and microarray analysis of gene expression was performed.
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Overall design |
Lungs were harvested from db/db mice exposed to air and ozone and injected with anti-IL-17A and isotype.
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Contributor(s) |
Shore SA, Mathews JA, Kasahara DI |
Citation missing |
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Submission date |
May 23, 2016 |
Last update date |
Apr 18, 2017 |
Contact name |
Stephanie A Shore |
E-mail(s) |
sshore@hsph.harvard.edu
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Organization name |
Harvard School of Public Health
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Department |
Environmental Health
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Lab |
Shore Lab
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Street address |
665 Huntigton Av Bld1 room 307
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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Samples (12)
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Relations |
BioProject |
PRJNA322558 |