 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 13, 2017 |
| Title |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This SuperSeries is composed of the SubSeries listed below.
|
| |
|
| Overall design |
Refer to individual Series
|
| |
|
| Citation(s) |
28416141 |
| Submission date |
May 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Gary Chung Hon |
| Organization name |
UT Southwestern
|
| Department |
OB/GYN
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (134)
|
| GSM2152025 |
K562 dCas9-KRAB, Lenti-sgRNA57_bc19 |
| GSM2152026 |
K562 dCas9-KRAB, Lenti-sgRNA59_bc33 |
| GSM2152027 |
K562 dCas9-KRAB, Lenti-sgRNA60_bc35 |
| GSM2152028 |
K562 dCas9-KRAB, Lenti-sgRNA62_bc37 |
| GSM2152029 |
K562 dCas9-KRAB, Lenti-sgRNA63_bc38 |
| GSM2152030 |
K562 dCas9-KRAB, Lenti-sgRNA80_bc24 |
| GSM2152031 |
K562 dCas9-KRAB, Lenti-sgRNA82_bc29 |
| GSM2152032 |
K562 dCas9-KRAB, not infected |
| GSM2175034 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 1 |
| GSM2175035 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 2 |
| GSM2175036 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 3 |
| GSM2175037 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 4 |
| GSM2175038 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 5 |
| GSM2175039 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 6 |
| GSM2175040 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 7 |
| GSM2175041 |
K562 dCas9-KRAB, Lenti-sgRNA pooled infection, beta-globin pilot, batch 8 |
| GSM2175042 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 1 |
| GSM2175043 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 2 |
| GSM2175044 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 3 |
| GSM2175045 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 4 |
| GSM2175046 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 5 |
| GSM2175047 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 6 |
| GSM2175048 |
K562 dCas9-KRAB, Lenti-sgRNA separate infection, beta-globin pilot, batch 7 |
| GSM2175049 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 1 |
| GSM2175050 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 2 |
| GSM2175051 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 3 |
| GSM2175052 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 4 |
| GSM2175053 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 5 |
| GSM2175054 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep2, batch 1 |
| GSM2175055 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep2, batch 2 |
| GSM2175056 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep2, batch 3 |
| GSM2175057 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep2, batch 4 |
| GSM2175058 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep2, batch 5 |
| GSM2175059 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 6 |
| GSM2175060 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 7 |
| GSM2175061 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 8 |
| GSM2175062 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 9 |
| GSM2175063 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 10 |
| GSM2175064 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 5 |
| GSM2175065 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 6 |
| GSM2175066 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 8 |
| GSM2175067 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 9 |
| GSM2175068 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 10 |
| GSM2175069 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 11 |
| GSM2175070 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 7 |
| GSM2175071 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 8 |
| GSM2175072 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 9 |
| GSM2175073 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 10 |
| GSM2175074 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 7 |
| GSM2175075 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 8 |
| GSM2175076 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 9 |
| GSM2175077 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 10 |
| GSM2175078 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 12 |
| GSM2175079 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 13 |
| GSM2175080 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 14 |
| GSM2175081 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 15 |
| GSM2175082 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 11 |
| GSM2175083 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 11 |
| GSM2175084 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 16 |
| GSM2175085 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 1 |
| GSM2175086 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 1 |
| GSM2175087 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 1 |
| GSM2175088 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 2 |
| GSM2175089 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 2 |
| GSM2175090 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 2 |
| GSM2175091 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 3 |
| GSM2175092 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 4 |
| GSM2175093 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 5 |
| GSM2175094 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 6 |
| GSM2175095 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, batch 7 |
| GSM2175096 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 3 |
| GSM2175097 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 4 |
| GSM2175098 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 5 |
| GSM2175099 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, batch 6 |
| GSM2175100 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 3 |
| GSM2175101 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, batch 4 |
| GSM2176650 |
K562 + MEF, 11 PCR1 cycles, 100 cells per ul, one-third beads for RT |
| GSM2176651 |
K562 + MEF, 13 PCR1 cycles, 100 cells per ul, one-third beads for RT |
| GSM2176652 |
K562 + MEF, 11 PCR1 cycles, 200 cells per ul, one-third beads for RT |
| GSM2176653 |
K562 + MEF, 13 PCR1 cycles, 200 cells per ul, one-third beads for RT |
| GSM2176654 |
K562 + MEF, 11 PCR1 cycles, 300 cells per ul, one-third beads for RT |
| GSM2176655 |
K562 + MEF, 13 PCR1 cycles, 300 cells per ul, one-third beads for RT |
| GSM2176656 |
K562 + MEF, 11 PCR1 cycles, 100 cells per ul, two-thirds beads for RT |
| GSM2176657 |
K562 + MEF, 13 PCR1 cycles, 100 cells per ul, two-thirds beads for RT |
| GSM2176658 |
K562 + MEF, 11 PCR1 cycles, 200 cells per ul, two-thirds beads for RT |
| GSM2176659 |
K562 + MEF, 13 PCR1 cycles, 200 cells per ul, two-thirds beads for RT |
| GSM2176660 |
K562 + MEF, 11 PCR1 cycles, 300 cells per ul, two-thirds beads for RT |
| GSM2176661 |
K562 + MEF, 13 PCR1 cycles, 300 cells per ul, two-thirds beads for RT |
| GSM2544700 |
K562, dCas9-KRAB, infected with sgRNA63 (rep1) |
| GSM2544701 |
K562, dCas9-KRAB, infected with sgRNA63 (rep2) |
| GSM2544702 |
K562, dCas9-KRAB, infected with sgRNA63 (rep3) |
| GSM2544703 |
K562, dCas9-KRAB, infected with sgRNA63 (rep4) |
| GSM2544704 |
K562, dCas9-KRAB, infected with sgRNA63 (rep5) |
| GSM2544705 |
K562, dCas9-KRAB, infected with sgRNA64 (rep1); treated with TNFalpha for 1h |
| GSM2544706 |
K562, dCas9-KRAB, infected with sgRNA64 (rep2); treated with TNFalpha for 1h |
| GSM2544707 |
K562, dCas9-KRAB, infected with sgRNA64 (rep3); treated with TNFalpha for 1h |
| GSM2544708 |
K562, dCas9-KRAB, infected with sgRNA64 (rep4); treated with TNFalpha for 1h |
| GSM2544709 |
K562, dCas9-KRAB, infected with sgRNA64 (rep5); treated with TNFalpha for 1h |
| GSM2544710 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 11 |
| GSM2544711 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 12 |
| GSM2544712 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 13 |
| GSM2544713 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 14 |
| GSM2544714 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 15 |
| GSM2544715 |
K562 dCas9-KRAB, WT vs TNFA treatment, biorep1, batch 16 |
| GSM2544716 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 3, batch 1 |
| GSM2544717 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 3, batch 2 |
| GSM2544718 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 3, batch 3 |
| GSM2544719 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 3, batch 4 |
| GSM2544720 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 3, batch 5 |
| GSM2544721 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 3, batch 6 |
| GSM2544722 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 10 |
| GSM2544723 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 11 |
| GSM2544724 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 1 |
| GSM2544725 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 2 |
| GSM2544726 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 3 |
| GSM2544727 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 4 |
| GSM2544728 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 5 |
| GSM2544729 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 6 |
| GSM2544730 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 7 |
| GSM2544731 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 8 |
| GSM2544732 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 9 |
| GSM2544733 |
K562 dCas9-KRAB, Lenti-sgRNA set A pooled infection, biorep 2, batch 12 |
| GSM2544734 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, biorep 2, batch 1 |
| GSM2544735 |
K562 dCas9-KRAB, Lenti-sgRNA set B pooled infection, biorep 2, batch 2 |
| GSM2544736 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, biorep 2, batch 1 |
| GSM2544737 |
K562 dCas9-KRAB, Lenti-sgRNA set C pooled infection, biorep 2, batch 2 |
| GSM2544738 |
K562 dCas9-KRAB, 10 sgRNAs targeting TAD2 SE2 enhancers, low MOI, batch 1 |
| GSM2544739 |
K562 dCas9-KRAB, 10 sgRNAs targeting TAD2 SE2 enhancers, low MOI, batch 2 |
| GSM2544740 |
K562 dCas9-KRAB, 10 sgRNAs targeting TAD2 SE2 enhancers, low MOI, batch 3 |
| GSM2544741 |
K562 dCas9-KRAB, 10 sgRNAs targeting TAD2 SE2 enhancers, low MOI, batch 4 |
| GSM2544742 |
K562 dCas9-KRAB, 10 sgRNAs targeting TAD2 SE2 enhancers, high MOI, batch 1 |
|
| This SuperSeries is composed of the following SubSeries:
|
| GSE81412 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Bulk RNA-Seq validation |
| GSE81747 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq of beta-globin locus (pooled infection) |
| GSE81748 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq of beta-globin locus (separate infection) |
| GSE81749 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq TNF-alpha treatment versus mock |
| GSE81750 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq of 15 SEs, 71 HSs, 241 sgRNAs |
| GSE81848 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Human/mouse mixing Drop-Seq experiments for K562 and MEFs |
| GSE96827 |
Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq of 10 enhancers in combination |
|
| Relations |
| BioProject |
PRJNA322853 |
|
|
|
|
 |
Less...
More...