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Series GSE8199 Query DataSets for GSE8199
Status Public on Jul 10, 2007
Title E18.5 Estrogen-related Receptor gamma Knockout Mouse Heart
Organism Mus musculus
Experiment type Expression profiling by array
Summary 3 ventricles from E18.5 male mice were pooled for each array. Three arrays per genotype.

Title: ERRγ Directs and Maintains the Transition to Oxidative Metabolism in the Post-Natal Heart

Abstract: At birth the heart undergoes a critical metabolic switch to transition from a predominant dependence on carbohydrates during fetal life to a greater dependence on postnatal oxidative metabolism. This remains the principle metabolic state throughout life; although pathologic conditions such as heart failure and cardiac hypertrophy reactivate components of the fetal genetic program to increase carbohydrate utilization. Disruption of the ERRγ gene, which is expressed at high levels in the fetal and postnatal mouse heart, blocks this switch resulting in lactatemia, electrocardiographic (ECG) abnormalities and death during the first week of life. Genomic ChIP-on-chip and expression analysis at E18.5 clearly identifies ERRγ as both a direct and indirect regulator of a nuclear-encoded mitochondrial genetic network that coordinates the postnatal metabolic transition. These findings reveal an unexpected and essential molecular genetic component of the oxidative metabolic gene program in the heart and highlight ERRγ in the study of cardiac hypertrophy and failure.
Keywords: ChIP-on-chip, electrocardiography, fetal gene program, OXPHOS, PGC-1a, sodium current, Single time point (E18.5) to compare genotype differences
 
Overall design Fetal mice were collected by caesarean secation. Hearts were stored in RNALater (Qiagen)
 
Contributor(s) Evans RM, Alaynick WA
Citation(s) 17618853
Submission date Jun 20, 2007
Last update date Feb 11, 2019
Contact name Ronald M Evans
E-mail(s) evans@salk.edu
Phone 858-453-4100
Organization name The Salk Institute for Biological Studies
Department Howard Hughes Medical Institute
Lab Gene Expression Laboratory
Street address 10010 North Torrey Pines
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM202772 E18.5 ERRg +/+ heart, biological rep 1
GSM202773 E18.5 ERRg +/+ heart, biological rep 2
GSM202774 E18.5 ERRg +/+ heart, biological rep 3
Relations
BioProject PRJNA101109

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8199_RAW.tar 75.5 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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