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Series GSE82319 Query DataSets for GSE82319
Status Public on Jun 01, 2018
Title PRKCI promotes immune suppression in ovarian cancer through YAP1
Organism Mus musculus
Experiment type Expression profiling by array
Summary A key feature of high-grade serous ovarian carcinoma (HGSOC) is frequent amplification of the 3q26 locus harboring PRKC-iota (PRKCI). Here, we show that PRKCI is also expressed in early fallopian tube lesions, called Serous Tubal Intraepithelial Carcinoma. Transgenic mouse studies establish PRKCI as an ovarian cancer specific oncogene and system level and functional analyses identify YAP1 as a downstream effector in tumor progression. Mechanistically, the oncogenic activity of the PRKCI-YAP1 axis relates in part to the upregulation of TNFα to promote an immune suppressive tumor microenvironment characterized by an abundance of myeloid-derived suppressor cells and inhibition of cytotoxic T cell infiltration. In human ovarian cancers, high PRKCI expression also correlates with high expression of YAP1 and low infiltration of cytotoxic T-cell. The PRKCI-YAP1 regulation of the tumor immunity provides a therapeutic strategy for highly lethal ovarian cancer.
Robust multi-array average (RMA) method was used with default options (with background correction, quantile normalization, and log transformation) to normalize raw data from batches using R/Bioconductor‘s affy package
Overall design Charterization of transcriptional alterations of tumors from a genetically engineered mouse model (GEMM) compared with fallopian tube of wild type mice.
Contributor(s) Sarkar S, Bristow CA, Chin L
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Submission date Jun 06, 2016
Last update date Jun 02, 2018
Contact name Sharmistha Sarkar
Organization name The University of Texas MD Anderson Cancer Center
Department Genomic Medicine
Street address 1515 Holcombe Boulevard
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL16368 [Mouse4302_Mm_ENTREZG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 16]
Samples (8)
GSM2188954 FT1 Control
GSM2188955 FT2 Control
GSM2188956 FT3 Control
BioProject PRJNA324608

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Supplementary file Size Download File type/resource
GSE82319_RAW.tar 43.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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