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Series GSE82323 Query DataSets for GSE82323
Status Public on Feb 09, 2017
Title Distinct skeletal muscle gene regulation from active contraction, passive vibration, and whole body heat stress in humans
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used a novel approach to study the acute effect of three physiologic stressors (active contractions, vibration, and systemic heat stress) in human skeletal muscle. Three hours after the completion of a dose of physiologic stress, we sampled the soleus (contraction and vibration) or vastus lateralis (heat) muscle and developed a unique gene expression signature for each stressor. We discovered repetitive active muscle contractions up regulated metabolic transcription factors NR4A3 (12.45 fold change), PGC-1α (5.46 fold change), and ABRA (5.98 fold change); and repressed MSTN (0.56 fold change). Heat stress repressed PGC-1α (0.74 fold change); while vibration induced FOXK2 (2.36 fold change). Vibration similarly caused a down regulation of MSTN (0.74 fold change), but to a lesser extent than active muscle contraction. Vibration induced FOXK2 while heat stress repressed PGC-1α (0.74 fold change) and ANKRD1 genes (0.51 fold change). These findings support a distinct gene regulation in response to heat stress, vibration, and muscle contractions. Understanding these responses may assist in developing regenerative rehabilitation interventions to improve muscle cell development, growth, and repair.
 
Overall design We analyzed skeletal muscle using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Partek Genomic Suites.
 
Contributor(s) Petrie MA, McHenry CL, Kimball AL, Yen C, Sharma A, Suneja M, Shields RK
Citation(s) 27486743
Submission date Jun 07, 2016
Last update date Feb 18, 2019
Contact name Richard Shields
Organization name The University of Iowa
Street address 1-252 Medical Education Building
City Iowa City
ZIP/Postal code 52242
Country USA
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (34)
GSM2190195 Skeletal Muscle Sample 1_contraction, control
GSM2190196 Skeletal Muscle Sample 2_contraction, experimental
GSM2190197 Skeletal Muscle Sample 3_contraction, control
Relations
BioProject PRJNA324650

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Supplementary file Size Download File type/resource
GSE82323_RAW.tar 673.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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