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Series GSE8253 Query DataSets for GSE8253
Status Public on May 19, 2008
Title Non-alcoholic Steatohepatitis following feeding of high polyunsaturated fat diets
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Most commonly used models of non-alcoholic steatohepatitis (NASH) are diets based on specific gene knockouts or represent extreme manipulations of diet. We have examined the effects of modest increased caloric intake and high dietary unsaturated fat content on the development of NASH in male rats using a model in which overfeeding is accomplished via intragastric infusion of liquid diets as a part of total enteral nutrition. Male Sprague dawley rats were fed diets 5% corn oil containing diets at 187 Kcal/kg3/4/d or fed 70% corn oil containing diets at 220 Kcal/kg3/4/d for a period of 3 weeks. Hepatic gene expression were assessed at the end of the study. Our results indicate that overfeeding of high unsaturated fat diets leads to pathological, endocrine and metabolic changes characteristic of NASH patients and is associated with increased oxidative stress and TNF-a.
Keywords: Steatosis and unsaturated fat
Overall design Two groups of male sprague dawley rats were fed liquid diets via total enteral nutrition.
Group 1, Control, Rats were fed diets containing 5% Corn oil at 187 Kcal/kg3/4/d for 3 weeks.
Group 2, NASH, Rats were fed diets containing 70% corn oil at 220 Kcal/kg3/4/d for 3 weeks.
Contributor(s) Baumgardner JN, Shankar K, Hennings L, Badger TM, Ronis MJ
Citation(s) 17947452
Submission date Jun 22, 2007
Last update date Feb 21, 2017
Contact name Kartik Shankar
Phone 501 364 2847
Fax 501 364 3161
Organization name University of Arkansas for Medical Sciences/Arkansas Childrens Nutrition Center
Department Pharmacology and Toxicology
Street address 1120 Marshall Street, Slot 512-20B
City Little rock
State/province AR
ZIP/Postal code 72202
Country USA
Platforms (1)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
Samples (6)
GSM204001 Control-1
GSM204002 Control-2
GSM204003 Control-3
BioProject PRJNA101201

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8253_RAW.tar 26.8 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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