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| Status |
Public on May 19, 2008 |
| Title |
Non-alcoholic Steatohepatitis following feeding of high polyunsaturated fat diets |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Most commonly used models of non-alcoholic steatohepatitis (NASH) are diets based on specific gene knockouts or represent extreme manipulations of diet. We have examined the effects of modest increased caloric intake and high dietary unsaturated fat content on the development of NASH in male rats using a model in which overfeeding is accomplished via intragastric infusion of liquid diets as a part of total enteral nutrition. Male Sprague dawley rats were fed diets 5% corn oil containing diets at 187 Kcal/kg3/4/d or fed 70% corn oil containing diets at 220 Kcal/kg3/4/d for a period of 3 weeks. Hepatic gene expression were assessed at the end of the study. Our results indicate that overfeeding of high unsaturated fat diets leads to pathological, endocrine and metabolic changes characteristic of NASH patients and is associated with increased oxidative stress and TNF-a.
Keywords: Steatosis and unsaturated fat
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| Overall design |
Two groups of male sprague dawley rats were fed liquid diets via total enteral nutrition.
Group 1, Control, Rats were fed diets containing 5% Corn oil at 187 Kcal/kg3/4/d for 3 weeks.
Group 2, NASH, Rats were fed diets containing 70% corn oil at 220 Kcal/kg3/4/d for 3 weeks.
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| Contributor(s) |
Baumgardner JN, Shankar K, Hennings L, Badger TM, Ronis MJ |
| Citation(s) |
17947452 |
| Submission date |
Jun 22, 2007 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Kartik Shankar |
| E-mail(s) |
shankarkartik@uams.edu
|
| Phone |
501 364 2847
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| Fax |
501 364 3161
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| Organization name |
University of Arkansas for Medical Sciences/Arkansas Childrens Nutrition Center
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| Department |
Pharmacology and Toxicology
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| Street address |
1120 Marshall Street, Slot 512-20B
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| City |
Little rock |
| State/province |
AR |
| ZIP/Postal code |
72202 |
| Country |
USA |
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| Platforms (1) |
| GPL85 |
[RG_U34A] Affymetrix Rat Genome U34 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA101201 |
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